摘要
目的监测2000-2005年间广州地区质粒和染色体介导的淋球菌对青霉素和四环素的耐药性及耐药株流行趋势。方法采用琼脂稀释法检测淋球菌对青霉素和四环素抗菌药物的最低抑菌浓度(MIC),用纸片酸度法测定质粒介导的产青霉素酶淋球菌(PPNG)。结果6年来共测定631株临床分离的淋球菌,检出PPNG132株(20.9%),阳性率从2000年的17.1%上升到2005年的23.7%(χ2=0.955,P>0.05);质粒介导的高度耐四环素淋球菌(TRNG)222株(35.2%),阳性率从2000年的20%上升到2005年的46.1%(χ2=11.94,P<0.05)。染色体介导的青霉素耐药率从2000年的76%上升到2005年的98.3%(χ2=12.94,P<0.05),染色体介导的四环素的耐药率介于70.7%～85.7%(χ2=3.246,P>0.05)。在2000-2005年期间,青霉素的MIC50和MIC90由1mg/L和2mg/L上升到4mg/L和>32mg/L,四环素的MIC50和MIC90由1mg/L和2mg/L上升到2mg/L和4mg/L,且都超过了耐药标准。结论广州地区近6年来质粒介导的淋球菌耐药株PPNG和TRNG增长速度较快,呈逐年上升趋势;染色体介导的淋球菌对青霉素和四环素耐药比率很高。
Objective To investigate the in vitro antibiotic susceptibility and resistant trends of Neisseria gonorrhoeae strains isolated in Guangzhou from 2000 to 2005. Methods The agar dilution method was used to determine the resistance of N. gonorrhoeae to penicillin and tetracycline plasmid-mediated resistant strains. The penicillinase- producing N. gonorrhoeae （PPNG） was determined by paper acidometric method. Results A total of 631 N. gonorrhoeae isolates were examined. There were 132 （20.9%） strains of PPNG and 222 （35.2%） strains of plasmid mediated resistance to tetracycline （TRNG）. During the period of 2000-2005, the prevalence of PPNG increased from 17.1% to 23.7%（Χ^2=0.955,P〉0.05 ）, while TRNG significantly increased from 20% to 46.1% （Χ^2=11.94,P〈0.05）. Positive rate of chromosome mediated resistance to penicillin increased from 76% to 98.3% （Χ^2=12.94, P〈0.05） and tetracycline ranged from 70.7% to 85.7% （Χ^2=3.246,P 〉0.05）. During 2000-2005, MIC50 and MIC90 of penicillin changed from 1 mg/L and 2 mg/L to 4 mg/L and 〉32 rag/L, while MIC50 and MIC90 of tetracycline changed from 1 mg/L and 2 mg/L to 2 mg/L and 4 mg/L. Their MIC50 and MIC90 were significantly higher than resistant standards. Condusion In the past 6 years, the prevalence of PPNG and TRNG had increased rapidly, and the proportions of chromosome mediated resistant isolates to penicillin and tetracycline were high in Guangzhou.
出处
《热带医学杂志》
CAS
2007年第1期60-62,共3页
Journal of Tropical Medicine
关键词
青霉素
四环素
淋球菌
耐药性
penicillin
tetracycline
Neisseria gonorrhoeae
resistance
