摘要
目的探讨关节软骨基质金属蛋白酶(matrix metalloproteinase,MMPs)-1、13mRNA及盘状结构域受体(discoidin domain receptor,DDR2)三者与关节软骨退变的关系。方法对58例关节软骨标本应用反转录-多聚酶链反应法(RT-PCR)测定MMP-1、13mRNA的表达,免疫组化法显色DDR2表达,进行图像分析研究。结果在轻度退变关节软骨中MMP-1、13呈低表达,在中重度退变软骨中MMP-1、13表达显著增高,在重度退变软骨中MMP-1,13有相对的下降,DDR2表达也呈现出与MMP-1、13相同的表达特点。结论MMP-1、13的过表达导致软骨Ⅱ型胶原的降解是软骨退变的重要原因,Ⅱ型胶原降解片段诱导DDR2表达增高进一步介导MMP-1、13的表达形成一个放大效应而加速了关节软骨退变。
Purpose To explore the relationship between the expression of matrix metalloproteinase（MMPs）- 1, 13 and discoidin domain receptor （DDR2） in the development of osteoarthritis. Methods The expressions of MMP-1, 13 mRNA by RT-PCR and the immunohistochemical expression of discoidin domain receptor （DDR2） were studied in 58 osteoarthritis cases. The image analysis explored the relationship between the expressions of MMP-1, 13 and DDR2 in the development of osteoarthritis. Results Low level expressions of MMP-1,13 were observed in early cartilage. With the development of osteoarthritis, the expressions of MMP-1,13 mRNA increased in moderate and end-stage osteoarthritis while relatively decreased in end-stage osteoarthritis. The immunohistochemical expression of DDR2 paralleled with the expression of MMP-1 and MMP-13. Conclusions MMP-1 and 13 play important roles in the development of osteoarthritis especially the degradation of Collagen Ⅱ. Collagen Ⅱ induced DDR2 accelerate the degeneration of cartilage by amplifing the expression of MMP-1,MMP-13.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2007年第1期126-128,共3页
Fudan University Journal of Medical Sciences
关键词
骨关节炎
基质金属蛋白酶
盘状结构域受体
osteoarthritis
matrix metalloproteinase
discoidin domain receptor
