摘要
血清作为疫苗生产中的主要原辅材料,很容易被支原体污染.为了更好、更准确地检测血清中支原体的污染情况,本试验对血清中支原体污染的检测及方法进行了分析.分别用直接培养法、DNA荧光染色法和PCR法对17批新生牛血清样品进行了支原体检测.直接培养法检测结果有15批支原体阴性,2批阳性,阳性率为11.8%;DNA荧光染色法和PCR法检测结果均为14批支原体阴性,3批阳性,阳性率为17.6%.以上结果表明培养法与DNA荧光染色法或PCR法之间存在明显差异.对血清支原体进行检测时,应用直接培养法和DNA荧光染色法或PCR法同时联合应用,以提高对血清中支原体检测的准确性.
To investigate the contamination situation of mycoplasmas in serum, the cultivatio, DNA fluorescent and polymerase chain reaction（ PCR） were used respectively for detection of mycoplasmas in 17 batches calf serum. The results showed that 2 batches out of 17 batches calf serum was contained by mycoplasmas in detection of cultivation. The positive rate was 11.8%. However, there were both 3 positive in methods of DNA fluorescent and PCR in the same sample. Positive rate was 17.6 %. It proved that there was significantly different between method of cultivation and DNA fluorescent or PCR. The cultivation combine with DNA fluorescent or PCR method for mycoplasmas detection can improve precision in batches calf serum.
出处
《西北民族大学学报（自然科学版）》
2006年第4期37-40,共4页
Journal of Northwest Minzu University(Natural Science)
关键词
血清
支原体
检测
serum
mycoplasma
detection
