摘要
目的探讨黄芪多糖(APS)对NOD小鼠T细胞亚群的免疫调节机制。方法观察APS组和对照组NOD鼠胰岛浸润淋巴细胞免疫组化、脾T淋巴细胞CD4+/CD8+亚群比值,RT-PCR法检测两组胰岛中IL-1β、IL-2、IL-6、IL-12、TNF-α和INF-γTh1型细胞因子的mRNA表达。结果APS可以降低NOD小鼠1型糖尿病的发病率,下降胰岛浸润炎症细胞及脾脏中的CD4+/CD8+亚群比值。与对照组相比,APS组胰岛IL-1β、IL-2、IL-6、IL-12、TNF-α和INF-γ的mRNA表达水平明显下调。结论APS能纠正NOD小鼠Th1型细胞/细胞因子的免疫失衡状态,预防1型糖尿病的发生。
[Objective] To investigate the immunoloregulation effects of Astragalus Polysaeeharide （APS） on T helper lymphocyte subgroups in non-obese diabetic （NOD） mice. [Methods] In APS group and control group, infiltrated lymphocytes of islets were analysed by immunocyto-chemistry, and CD4^＋/CD8^＋ ratio of T lymphocytes from spleen was analysis by flow cytometer. The mRNA expression of IL-1β, IL-2, IL-6, IL-12, TNF-α, INF-γ of islets were detected with RT-PCR in two groups. [Results] Compared with control group, CD4^＋/CD8^＋ ratio of T lymphocytes from spleen was lower, and CD4^＋ T lymphocytes of islets were less, and the mRNA expression of IL-1β, IL-2, IL-6, IL-12, TNF-α,INF-γ was lower in APS group. [Conclusions] It proves the preventive effects of Astragalus Polysaccharide on the onset of type 1 DM by correcting the immunal imbalance of Thl subgroups and its cytokines in NOD mice.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第1期28-31,35,共5页
China Journal of Modern Medicine
关键词
黄芪多糖
1型糖尿病
免疫
细胞因子
Astragalus Polysaccharide
diabetes mellitus, type 1
immunology
cytokines
