期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

16S rRNA定量分析技术对绵羊瘤胃固相纤维降解菌的研究 被引量：1

Study on sheep rumen solid cellulolytic bacteria by 16S rRNA quantitative analytical techniques
在线阅读 下载PDF
导出
摘要 本试验选择3只安装永久性瘤胃瘘管、体重25￣30kg的羯羊,使用特异性16SrRNA目标探针进行斑点杂交研究绵羊瘤胃固相纤维降解菌的动态变化规律。结果表明:3种纤维分解菌的RNA占总菌数RNA的15.76%;在各时间点产琥珀酸拟杆菌的相对含量均高于黄化瘤胃球菌和白色瘤胃球菌相对含量。 Three 25 - 30 kg sheep with ruminal cannula were used to study variety disciplinarian of solid attached ruminal predominant cellulolytic bacteria.Three cellulolytic bacteria was quantified by dot-blot hybridisation using specific 16S rRNA-targeting probes.The sum of RNA of the three main cellulolytic bacterial species represented on average 15.76 % of the total bacterial RNA.In all samples,the relative population size of F.succinogenes was higher than that of R.albus and R.flavefaciens.
作者 薛丰 刘大程 王洪荣 卢德勋 高民
机构地区 内蒙古农业大学动物科学与医学学院 扬州大学动物科技学院 内蒙古农牧科学院动物营养所
出处 《中国饲料》 北大核心 2007年第1期38-40,43,共4页 China Feed
基金 国家自然科学基金资助项目"瘤胃微生物种群对纤维物质降解的机理及其相互作用研究" 项目编号:30460095
关键词 16S rKNA 纤维分解菌 斑点杂交 16S rRNA eellulolytic bacteria dot-blot hybridisation
分类号 S816.17 [农业科学—饲料科学]
  • 相关文献

参考文献9

  • 1Chen J Q,Weimer P J.Competition anong three predominant ruminal cellulolydc bacteria in the absence or presence of non-cellulolytic bacteria[J].Microbiology,2001,147:21 - 30.
  • 2Kolie S,Kobayashi Y.Development and use of competitive PCR assays for the rumen cellulolytic bacteria:Fibrobacter succiogenes、Ruminoccoccus albus and Ruminoccoccus fiavefaciens[J].FEMS Microbiol Let,2001,204:361 -366.
  • 3Krause D O,Russell J B.An rRNA-approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid dearnination.[J].Appl Environ Microbiol,1996,62:815 - 821.
  • 4McSweeney C S,Palmer B,Bunch R,et al.Effect of the tropical forage calliandra on microbial protein synthesis and ecology in the rumen[J].Journal of Applied Microbiology,2001,90:78 - 88.
  • 5Michalet-Doreau B,Fernandez I,Fibrolytic,et al.Fibrolytic activities and cellylo1ytic bacterial community structure in the solid and liquid phases of rumen contents[J].Nutr Dev,2001,41:187 - 194.
  • 6Michalet-Doreau B,Fernandez I,fonty G.A Comparison of enzymatic and molecular approaches to characterize the cellulolytic microbial ecosystems of the rumen and the cecum[J].J Anim Sci,2002,80:790 - 796.
  • 7Odenyo A A,Mackie R I,Stahl D A.The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria:development of probes for ruminococcus species and evidence for bacteriocin production[J].Appl Enuivon Microbiol,1994,60:3688 - 3696.
  • 8Shi Y,Weimer P J.Competition for cellobiose among three predominant ruminal cellulolytic bacteria under substrate-excess and substrate-limited conditions[J].Appl Environ Microbiol,1997,63:743 - 748.
  • 9Weimer P J,Waghorn G C,Odt C L,et al.Effect of diet on population of three species of ruminal cellulolytic bacteria in lactating dairy cows[J].J Dairy Sci,1999,82:122 - 134.

同被引文献17

  • 1Akin D E,Amos H E.Rumen bacterial degradation of forage cell walls incestigated by electron microscopy[J}.Appl nficrobiol, 1975,29 : 692 - 701.
  • 2Akin D E,Burdick,Michaels G E.Rumen bacterial interrelationship with plant tissue during degradation revealed by transmission electron microscopy[J]. Appl microbiol, 1974,27:1149 - 1156.
  • 3Akin D E,Gordon G L R,Hogan J P.Rumen bacterial and fungal degradation of Digitaria pentzii growth with or without sulfur[J].Applied and Envioronmental Microbiology, 1983,46 : 738 - 748.
  • 4Akin D E.Evaluation by electron microscopy and anaerobic culture of types of rumen bacteria associated with digestion of forage cell wall[J].Applied and Envioronmental Microbiology, 1980,39 : 242 - 252.
  • 5Bhat S,Wallace R J,Rskov E R.Adhesion of Cellulolytic Ruminal Bacteria to Barley Straw [J].Applied and Environment Microbiology, 1990,56 (9): 2698 - 2703.
  • 6Chen J,Stevenson D M,Weirner P J.Albusin B,a bacteriocin from the rummal bacterium Ruminococcus albus-7 that inhibits growth of Ruminococcus flavefaciens [J].Applied and environruental microbiology,2004,70 (5) :3167 - 3170.
  • 7Craig W M,Broderick G A,Ricker D B.Quantitation microorganisms associated with the particulate phase ofruminal ingesta[J].J Nutr, 1987,117:56 - 64.
  • 8Gong,Forsberg C W.Factors Affecting Adhesion of Fibrobacter succmogenes subsp:Succinogens S85 and adherence-Defective Mutants to Cellulose [J].Applied environment Microbiology, 1989,55 (12) : 3039 - 3044.
  • 9Koike S,Pan J,Kobayashi Y,et al.Kinetics of in sacco fiber-attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR [J].J Diary Sci, 2003,86 : 1429 - 1435.
  • 10Kudo H,Cheng K J,Costerton J W.Electron microscopy study of the methyl-cellulose mediate detachment of cellulytic tureen bacteria from cellulose fibers[J].Can J Microbiol, 1987,33:267 - 272.

引证文献1

二级引证文献1

中国饲料
2007年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部