摘要
目的研究正常人乳腺上皮细胞原代培养的可行性,以及激素对其增殖的影响。方法从乳房区段切除术切除组织中获取正常人乳腺组织,用胶原酶溶液消化乳腺组织分离得到较纯净的上皮细胞,用烧灼法结合酶消化法解决细胞纯化问题。细胞形态观察和电镜检测鉴定细胞。结果正常人乳腺上皮细胞的原代培养是可行的。1×10-5的雌二醇和孕酮混合液可以促进细胞增殖。
Objective To investigate the feasibility of primary culture of nontumorigenic human mammary epithelial cells, and the effect of proliferation due to hormone. Methods Normal human mammary tissue was taken from mammary segment ectomy, and the tissue was digested by collagenase type I. Epithelial deputation was carried out by integration of burning and enzyme digestion. The cells we acquired were identified by observation of cell morphology by light and Transmission Electron Microscopy （TEM）. Results It is feasible to culture nontumorigenic human mammary epithelial cells. β-estradiol and progesterone at 1 × 10^-5 g/L can promote the proliferation of the normal human mammary epithelial cells.
出处
《基础医学与临床》
CSCD
北大核心
2007年第1期81-84,共4页
Basic and Clinical Medicine
基金
上海市重点学科中医外科学(龙华医院)建设资助项目(T0304)
关键词
人乳腺上皮细胞
原代培养
human mammary epithelial cell
primary culture
