摘要
目的动态观察趋化因子Fractalkine(FKN)在内毒素(LPS)所致急性肺损伤(ALI)大鼠肺组织内表达水平的变化,和参芪扶正注射液对其的影响。方法经尾静脉注射LPS(4 mg／kg)建立ALI大鼠模型。将42只大鼠随机分为7组,每组6只:正常对照组、模型1、2、4 h 3个时相组和参芪扶正注射液1、2、4 h 3个时相组。采用酶联免疫吸附测定法(ELISA)、逆转录多聚酶链反应(RT-PCR)等方法,观察ALI大鼠模型肺组织病理学改变、肺湿干重比率(W／D)及血清肿瘤坏死细胞因子α(TNF-α)变化,检测肺组织FKN mR- NA的表达,同时观察参芪扶正注射液对上述指标的影响。结果(1)模型1、2、4 h组肺组织病理改变明显,肺损伤程度重,以2 h组最为显著,参芪扶正注射液能减轻ALI大鼠肺组织病理变化。(2)模型3个时相组肺W／D均明显增加,参芪扶正注射液能降低各时相组肺W／D值。(3)模型3个时相组血清TNF-α均明显增高,于2 h点达到最高值,参芪扶正注射液能显著降低各时点血清TNF-α水平(P<0．05)。(4)正常大鼠肺组织有FKN mRNA的表达,模型3个时相组肺组织FKN mRNA表达较正常组明显增加,在2 h时点达最高值,参芪扶正注射液能降低ALI大鼠肺组织FKN mRNA的表达(P<0．05)。FKN mRNA的表达与血清TNF-α水平呈正相关。结论早期使用参芪扶正注射液能改善ALI肺组织病理改变,减轻肺水肿,降低血清TNF-α水平,下调肺组织FKN mRNA的表达,对内毒素致急性肺损伤实验大鼠具有保护作用。
Objective To observe dynamically the Fraetalkine （FKN） expression in lung tissue of rats with lipopolysaccharide （LPS）-induced acute lung injury （ALI） and the effect of Shenqi Fuzheng Injection （SFI） on it. Methods Rat model of ALI was established by intravenous injection of 4 mg/kg of LPS. Forty-two Wistar rats were randomly divided into 7 groups, the normal group, the model group and the SFI group, the latter two were separated respectively into three time phases （the 1 h, 2 h and 4 h after modeling ） groups, 6 rats in each group, Pathological changes and wet/dry weight ratio （W/D） of lung were observed, serum TNF-α and FKN mRNA expression in the lung tissue were examined by ELISA and RT-PCR respectively. Results Severe pathological changes of lung presented in the model groups of all three time phases with a higher W/D ratio, as well as increased serum TNF-α level and FKN mRNA expression in lung tissue. The peak of abnormality of serum TNF-α level and FKN mRNA expression was shown in the 2 h time phase group. All the above-mentioned abnormal changes were alleviated after treatment in the SFI group （P〈0.05）. In addition, the level of FKN mRNA expression was found to be positively correlated to the serum TNF-α concentration. Conclusion SFI treatment in early stage could relieve the pathological changes and edema in lung tissue, decrease serum TNF-α and down-regulate FKN mRNA expression, playing a protective role in LPS-induced ALI rats.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2007年第1期55-59,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家中医药管理局青年基金(No.04-05JQ03)
