摘要
目的探讨乳腺癌组织14-3-3 sigma 基因甲基化状态和表达情况。方法用甲基化特异性聚合酶链反应(MSP)方法,分别检测40例乳腺癌患者肿瘤组织和18例乳腺良性病变组织的14-3-3 sigma 基因甲基化状态、并用逆转录(RT)-PCR 和免疫印迹法(WB)分别从 mRNA 和蛋白质水平检测14-3-3 sigma 基因的表达情况。结果在40例乳腺癌患者肿瘤组织中,MSP 法检测出34例14-3-3 sigma 基因甲基化,甲基化率达85%;而 RT-PCR 为12.5%(5/40);WB 法显示,80%患者乳腺癌组织存在14-3-3 sigma 蛋白表达缺失(32/40);在34例 MSP 甲基化患者中,有30例 RT-PCR 和 WB同时显示阴性(88%)。18例良性疾病患者病变乳腺组织均未检出14-3-3 sigma 基因甲基化,而 RT-PCR 和 WB 同时也证明14-3-3 sigma 基因在乳腺上皮细胞均有表达。提示乳腺癌时14-3-3 sigma 基因高度甲基化,且可能与14-3-3 sigma 基因表达缺失密切相关。结论 14-3-3 sigma 基因甲基化及其表达缺失是乳腺癌的经常性事件,14-3-3 sigma 基因甲基化可能与其基因表达缺失有关。
Objective To observe the methylation and expression of 14-3-3 signm gene in human breast cancer. Methods 40 breast cancer tissues and 18 mammary gland tissues with benign lesions were analyzed by methylation specific PCR （MSP）, RT-PCR, and Western-blot （WB） so as to detect the methylation status and expression of 14-3-3 signm mRNA or protein. Results Methyhttlon of 14-3-3 sigma gene was detectable in 85% （34/40） of patients with breast cancers. RT-PCR showed negative in 12. 5% of breast cancers（5/40）, WB also indicated that 14-3-3 sigma was not detected in 32 of 40 breast carcinomas （80%）. Furthermore, both RT-PCR and WB were negative in 30 of 34 positive cases by MSP. While methylation of 14-3-3 sigma was not detectable and its expression was demonstrated by RT-PCR and WB among 18 cases of benign breast diseases. These evidences proposed that methylation of 14-3-3 signm gene had great relevance with its silence. Conclusion Methylation and loss of expression in 14-3-3 sigma gene were high frequent events in breast cancers. And methylation of 14-3-3 sigma gene might be related to its loss.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2007年第1期41-43,共3页
Chinese Journal of Laboratory Medicine
基金
安徽省教育厅自然科学基金(2006KY127C)
安徽省教育厅高校青年教师基金(2006jq1177)
关键词
乳腺肿瘤
甲基化
基因
Breast neoplasm
Methylation
Genes
