摘要
目的:观察实验性重症减压病(DCS)过程中兔血细胞成分、血管内皮标记物及肺组织的变化,比较存活组与死亡组的异同。方法:14只新西兰白兔,在0.55 MPa下停留35 m in,再升至0.7 MPa停留35 m in,于4m in内匀速减压出舱。检测加压前、减压后兔血液中白细胞(WBC)、血小板(PLT)计数及血浆内皮素-1(ET-1)等指标。观察存活与死亡兔肺组织形态变化,比较存活动物与死亡动物以上指标变化的特点。结果:经历快速减压后,8只兔于减压后30m in内死亡,6只存活且观察24 h后未遗留任何减压病症状。死亡组兔加压前白细胞总数为(11.13±2.37)×109/L,明显高于存活组(8.87±2.11)×109/L,死亡组兔减压过程PLT减少量为(273±63)×109/L,明显大于存活组(148±63)×109/L。减压后死亡与存活兔肺组织均可见部分肺泡塌陷、间质水肿、局限性肺气肿、微循环内可见多种栓子、肺呼吸膜增厚等多种病理改变,而死亡组兔病变程度明显大于存活组兔。结论:实验性重症减压病动物体内出现循环张力增高,多种栓子形成,肺组织呼吸膜增厚,表面活性物质耗尽等病理生理过程,机体对减压刺激反应剧烈,相应代偿能力差的动物最终死亡,反之则可存活。
Objective： To observe the change of blood cell, marker of vascular endothelium and lung of rabbits during experimental severe decompression sickness （DCS） and to compare each parameter between survival group and dead group. Methods： 14 rabbits were put into the chamber. They stayed for 35mins under 0.55 MPa, and another 35 mins under 0.7 MPa, then decompressed to 0.1 MPa in 4 minutes. Samples were drew before the compression and after decompression for testing count of white blood cell（WBC）, neutrophilic leukocyte, lymphocyte and platelet（PLT） and the concentration of ET-1 and the vWF. The tissue of lung of all the rabbits was observed under light and electron microscope. The changes of above parameters of survival rabbits were compared with that of the dead. Results： After rapid decompression, there were 8 rabbits died within 30 minutes, while the other 6 rabbits survived without any symptom of decompression sickness after 24 hours observation. Before the compression, the total count of WBC of dead rabbits was × （ 11.13 ±2.37） × 10^9/L, which was higher the that of survival rabbits, （8.87 ± 2. 11 ） × 10^9/L. After decompression, the decrease of PLT of dead group, （273 ± 63 ） × 10^9/L,was more than that of survival group, （ 148 ± 63 ） × 10^9/L. Ttissuedamage of lungs of both dead and survived rabbits was observed as partial alveoli collapse, interstitial edema, local emphysema, various emboli in microcirculation and thickening of respiratory membrane. The damage of lung of dead rabbits was much worse than that of the survival. Conclusion ： Experimental DCS induced the increasing of circulation tension, WBC obturation, various emboli, thickening of respiratory membrane, consumption of surface active substance in the body of rabbits. Those rabbits who reacted too severe and had bad compensation ability would die, on the contrary, those rabbits who had a good compensation could survive.
出处
《医学研究生学报》
CAS
2007年第1期28-31,I0010,共5页
Journal of Medical Postgraduates
基金
全军医药卫生科研基金资助项目(批准号:01MA052)
关键词
减压病
中性粒细胞
淋巴细胞
血小板
Decompression sickness
Neutrophilic leukocyte
Lymphocyte
Platelet
