摘要
以大豆品种“晋豆8号”为材料,采用组织培养方法研究了Ca(NO_3)_2对盐胁迫下大豆离体胚再生植株有机物质积累、膜脂过氧化和保护酶系统的影响.结果表明,适宜浓度的Ca(NO_3)_2处理,可促进一定盐胁迫下大豆再生植株内游离脯氨酸和可溶性糖的积累,以缓解盐胁迫造成的渗透伤害,并可促进保护酶系统超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,减少膜质过氧化产物丙二醛(MDA)含量,降低相对膜造性,减轻盐对膜的伤害.低盐胁迫时以Ca(NO_3)_2 15mM、高盐胁迫时以 Ca(NO_3)_2 10mM效果最佳.
The effect of Ca (NO3)2 on membrane lipid peroxidation and enzyme system for membrane protection in soybean regenerated plantlets from embryoes in vitro was studied by means of tissure culture. The result showed that Ca(NO3)2 promoted the activities of SOD,CAT which belong to the enzyme system for membrane protection of soybean plantlets under certain salt stress ,decreased the relative permeability of cell membrane. Therefore, relieving salt injury to membrane. The best treatment concentration of Ca (NO3)2 were 15mM and 20mM under lower salt atress and higher salt stress respectively. In addition, treatment of suitable Ca (NO3)2 concentration promoted accumulation of proline and suger, so as to revive salt injury.
基金
山西省自然科学基金资助项目
关键词
大豆
盐胁迫
植株再生
Soybean, Ca (NO3)2, Salt stress, Free proline, Membrane liptd peroxidation, protective enzyme system
