摘要
为了研究庚型肝炎病毒(HGV)在我国的感染状况,依照对HGV氨基酸序列的亲水性和抗原决定簇位点分析,采用固相法合成了HGV不同区段的4条多肽,以此为抗原建立了检测抗HGV-IgG的间接酶联免疫吸附试验。检测57份非甲-戊型肝炎病人血清,抗HGV-IgG阳性者20份,阳性检出率为35.09%(20/57),HGV RNA阳性者14份,阳性率为24.56%(14/57)。检测甲型肝炎病人血清30份,阳性率为3.33%(1/30),乙型肝炎病人血清10份,阳性率为10%(1/10),丙型肝炎病人血清46份,阳性率为8.70%(4/46),证明在我国的病毒性肝炎患者中有较高的HGV感染率,在病毒性肝炎的鉴别诊断中应引起注意。同时,HGV主要经血液传播,应高度重视其由血源传播的危险性。
According to the HGV amino acid sequence, 4 peptides from different regions were selected based on the computer analysis of the hydrophility and antigenic epitopes and were synthesized by the conventional solid phase method. With the synthetic peptides, an indirect ELISA was developed to detect anti-HGV IgG. Among 57 sera from non A-E hepatitis patients, 20 were positive for anti-HGV IgG, the positive rate was 35.09 % (20/57), 14 were positive for HGV RNA, the positive rate was 24.56% (14/57). We also detected 30 sera from HA, 10 from lib and 46 from HC, the positive rate for anti-HGV lgG were 3.33%, 10% and 8.70% respectively. The results showed that the coinfection rate is relatively high in viral hepatitis patients in our country. Therefore HGV infection should be given attention to in the differential diagnosis of hepatitis. And we should also deal seriously with HGV spread by transfusion.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
1996年第4期371-373,共3页
Chinese Journal of Experimental and Clinical Virology
