摘要
利用植物细胞培养技术生产次生代谢产物近年来取得很大进展,但如何增加目的产物产量使该技术实现工业化生产是亟待解决的难题。从筛选高产细胞系、优化培养环境、调节营养水平、前体饲喂、诱导子添加及β-环糊精使用等方面综述了国内外最新的增产策略。另外毛状根培养、固定化培养和生物转化研究正成为工业化生产次生代谢产物的重要技术来源。
Great advance have been achived in producing secondary metabolities by plant cell culture. But it is a problerm how to enchance production of metabolities of interest and realize industrial production. This review deals with some recently strategies to increase metabolities production, including screening of high growth cell line, optimizing the culture environment, regulating nutrient level, precursor feeding, elicitor adding, using β-Cyclodextrin etc. In addition, hairy root culture, immotilization and biotransformation are promising impartant sources for the production of high-value secondary metabolities of industrial application.
出处
《云南农业大学学报》
CAS
CSCD
2007年第1期1-7,共7页
Journal of Yunnan Agricultural University
基金
沈阳市科学技术基金资助(1053125-1-51)
关键词
次生代谢物
植物细胞培养
毛状根培养
固定化
生物转化
secondary metabolities
plant cell culture
hairy root culture
immobilication
biotrans formation
