摘要
目的:观察肝癌中医证型与野生型p53mRNA、N-ras表达相关性。方法:选择肝癌脾虚证36例和湿热证患者24例,应用原位杂交和免疫组化方法分别检测其肝癌组织野生型p53mRNA和N-ras蛋白表达,比较2组之间的差异。结果:脾虚证组野生型p53mRNA表达水平显著低于湿热证组(P<0．05);脾虚证组N-ras蛋白表达水平略低于湿热证组(P>0．05)。结论:野生型p53mRNA表达与旰癌中医证型有关,野生型p53 mRNA低表达是区别脾虚证和湿热证的特征之一。
Objective: To study the correlation of TCM syndrome differentiation and p53 mRNA and N - ras protein expression of wild type liver cancer. Methods: 36 cases liver cancer with spleen - deficiency syndrome (SDS) and 24 cases with damp - heat syndrome (DHS) were chosen, the p53 mRNA and N - ras protein expression of liver cancer tissue of wild type were determined by hybridism in situ and immunohistochemistry individually and the differences between two groups were compared. Results: The expression level of p53 mRNA of SDS was marked lower than DHS in wild type (P < 0. 05); the expression level of N - ras protein of SDS was slightly lower than DHS ( P > 0. 05). Conclusion: The expression of p53 mRNA related with TCM syndrome differentiation, the low expression level of p53 mRNA is one of characteristic to distinguish the SDS and DHS.
出处
《新中医》
CAS
北大核心
2007年第1期85-86,共2页
New Chinese Medicine
基金
广东省中医药局科研课题(编号:403046)
广东省自然科学基金项目(编号:035177)
