摘要
目的：测定黑石耳（Ｄｅｒｍａｔｏｃａｒｐｏｎｍｉｎｉａｔｕｍ，ＤＥＭ）多糖的组成、分子量及苷键的连接方式。方法：利用气相色谱法测定多糖的组成，凝胶过滤法测定分子量，Ｓｍｉｔｈ降解及高碘酸氧化确定苷键的连接方式。红外光谱测定苷键类型。结果：ＤＥＭ多糖由葡萄糖聚合而成，平均分子量为１．８×１０６。高碘酸氧化，Ｓｍｉｔｈ降解有甲酸、丙三醇、丁四醇生成。结论：ＤＥＭ多糖主要由α（１→４）、α（１→６）苷键连接而成的葡聚糖。
OBJECTIVE:Todeterminethecomponentsugars,themolecularweight,andtheformsofglucosidiclinkageofDEM(Dermatocarponminiatum)polysaccharide.METHODS:Thecomponentsugarwasdeterminedbygaschromatographyanalysis.Itsmolecularweightwasmeasuredbygelfiltration.Theformsofglucosidiclinkageweredeterminedaccordingtoperiodateoxidation,Smithdegradation,andIR.RESULTS:Themeanmolecularweightwasestimatedtobe1.8×106.AstheresultofperiodateoxidationandSmithdegradation,formicacid,glyc-erolanderythritolwereproduced.CONCLUSION:DEMpolysaccharide,aglucan,wasonlycomposedofD-glucoseunitsjoinedbyα-1,4andα-1,6glucosidiclinkages.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1996年第9期520-522,共3页
Chinese Pharmaceutical Journal
关键词
黑石耳
多糖
葡聚糖
Dermatocarponminiatum(L.)Mann.,polysaccharide,glucan,lichen
