摘要
目的:探讨不同条件对胎鼠海马神经干细胞的增殖与分化的影响。方法:从胎鼠海马获取神经干细胞;新生鼠坐骨神经获取雪旺细胞。培养成功后对细胞进行鉴定。将提取的神经干细胞放在不同的培养条件下:雪旺细胞与神经干细胞共培养、DMEM/F12+bFGF+EGF及DMEM/F12进行比较,观察其增殖和分化的情况。结果:含雪旺细胞的共培养组神经干细胞生长分化得最好,bFGF+EGF组次之,DMEM/F12组生长的最差;共培养组细胞团之间建立了良好的突触联系,尤其是远距离的细胞间更为明显。此现象在bFGF+EGF组及DMEM/F12组则没有出现,且该两组生长速度也较共培养组差。结论:雪旺细胞能更好的促进神经干细胞的生长、增殖和分化,它为神经干细胞提供了与活体内相似的环境。
Objective： To observe the influence of different conditions on proliferation and differentiation of neural stem cells（NSCs）. Methods： We cultured NSCs by taking the cells in hippocampus of fetal rats and the schwann cells by taking cells in ischiadicus in neogenesis rat, and used the nestin and S-100 to indentify NSCs and SCs. We cocultured SCs and NSCs, observe the comparison of the change of proliferation and differentiation of neural stem cells by different groups： the cocultured group , the DMEM/F12＋bFGF＋EGF group and the DMEM/F12group. Result： We found that NSCs after the cocultured groups seperated in a long distance had already combined each other very well with synapsis. The phenomena mentioned above did not emerge in the other groups. In the bFGF＋EGF group, NSCs reached the growth peak 6 days after being cultured, they did not generate synapsis until they have already passed continuously for four generations or more and the number of connection between NSCs is very small and it is very difficult for NSCs seperated in a long distance to combine each other with synapsis. Their growth speed and synapsis establishment are very different from that of NSCs with SCs. Conclusion： SCs provide NSCs with the neural nutrition surpporting, which means that SCs provide NSCs with the growth condition in vitro, which is similar to that in vivo by reproducing nerve growth factors and expression contents and make NSCs grow and multiply in a good phase.
出处
《广西医科大学学报》
CAS
北大核心
2006年第6期904-906,共3页
Journal of Guangxi Medical University
基金
2005年吉林省科技发展计划项目(No.200505204)
关键词
神经干细胞
培养
雪旺细胞
胎鼠
neural stem cells
culture
schwann cells
rat embryo
