摘要
利用溶剂提取法通过正交试验对冬青叶中的黄酮进行了提取,采用DPPH法对其抗氧化活性进行测定,用紫外分光光度法测定提取液中总黄酮含量;结果显示提取黄酮的最佳工艺条件为:乙醇溶液70%,浸提时间18h,回流时间4h,提取液的提取率为1.13%,抗氧化活性最高为97.74%,且提取液中总黄酮的含量与抗氧化活性成正比。
The best condition for extract flavones in holly leaf was determined by orthogonal test. The UV spectrophotometer was used to determine the content of flavones of extraction, DPPH assay method was used to determine the antioxidant activities of extraction. The best condition for extracting flavones in holly leaf was： extraction 18h with 70% concentration of ethanol, and reflux 4h in total, extraction production rate was 1.13%, the best antioxidant properties was 97.74%, and the content of flavones was directly proportional to the antioxidant activities.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第12期423-426,共4页
Food Science
基金
江苏省海洋生物技术重点实验室资助项目(2005HS008)
淮海工学院引进人才启动基金资助项目(KK01060)
关键词
冬青
黄酮
提取
活性测定
holly
flavones
extract
activity determination
