摘要
目的:同步联检急性冠状动脉(冠脉)综合征患者循环血中10种蛋白标志物浓度并探讨其临床意义。方法:运用蛋白芯片技术联检经冠脉造影及临床表现证实为急性冠脉综合征患者104例(分为急性心肌梗死组54例、不稳定性心绞痛组50例)及正常对照组50例血清或血浆中10种蛋白标志物水平,选择其中3种蛋白标志物与经典的酶联免疫双抗体夹心吸附实验(ELISA法)对照。结果:急性心肌梗死组和不稳定性心绞痛组血清中基质金属蛋白酶-9(MMP-9)、可溶性CD40L(sCD40L)、肌钙蛋白I(cTnI)、C反应蛋白(CRP)、心脏型脂肪酸结合蛋白(H-FABP)、白细胞介素-6(IL-6)及血浆中内皮素-1(ET-1)浓度,与正常对照组比较,差异有显著性(P＜0.01);急性心肌梗死组血清中H-FABP、cTnI含量明显高于不稳定性心绞痛组,差异有显著性(P＜0.01)。CRP与IL-6和sCD40L与可溶性血管细胞粘附分子-1(sVCAM-1)、MMP-9之间的直线相关分析结果发现,CRP与IL-6显著正相关(r=0.961,P＜0.01);sCD40L与sVCAM-1显著正相关(r=0.644,P＜0.01), sCD40L与MMP-9无相关性(r=0.158,P＞0.05)。采用蛋白芯片法与ELISA法检测急性冠状动脉综合征患者血中的3种蛋白标志物,结果显示H-FABP、sCD401的含量,差异无显著性(P＞0.05),而MMP-9差异有显著性(P＜0.01)。结论:心血管蛋白芯片技术不失为一种综合评估急性冠脉综合征蛋白标志物的有力工具。急性冠脉综合征患者循环中10种蛋白标志物水平异常,可作为急性冠脉综合征发生、发展过程中分子水平的标志物群。
Objective：To examine the concentration of 10 protein markers in patients with acute coronary syndrome （ACS）with extra protein chips and to explore its clinical significance.
Methods ：Combination detection of ~erum and plasma levels of 10 cytokines was performed in a prospective study of 104 patients with documented ACS and 50 comrols.
Results：The levels of serum MMP-9, sCD40L, sVCAM-I, CRP, IL-6, H-FABP, cTnI, and plasma ET-1, vpro-BNP and IL-8 in acute myocardial infarction （AMI）, unstable angina pectoris （UAP） patients were much higher than those of the controls （P 〈0.01 ）. The levels of serum cTnI and H-FABP in the AMI patients were much higher than those of UAP patients（ P 〈 0.01 ）. There was a close relationship both between the level of CRP and that of IL-6 （ r = 0. 961, P 〈 0.01 ） and between the level of sCD40L and that of sVCAM-1 （r =0.644, P 〈0.01 ）. There was no relationship between the level of sCIMOL and that of MMP-9 （r = 0. 158, P 〉 0.05 ）. The comparison of the two methods showed that there was no significant difference between serum H-FABP and sCD40L in patients with ACS.
Conclusions ： It showed that the lew;ls of 10 protein markers in ASC patients are abnormal. The results can be used as detection markers of ACS at a molecular level.
出处
《中国循环杂志》
CSCD
北大核心
2006年第6期426-430,共5页
Chinese Circulation Journal
