摘要
目的:分析心理应激状态下中小负荷运动大鼠体内β-内啡肽和白细胞介素1含量变化。方法:实验于2003-09/11在扬州大学体育学院动物实验室进行。选用健康雄性SD大鼠56只,随机均分为7组:①对照组:不给予任何刺激。②电击组:采用自制的电击笼对其电击。电流为1.0～2.0mA,电压30V,周期100s,间歇90s,每次电击持续30min,隔天1次,连续2周。③心理应激组:旁观电击组大鼠遭受电击的过程,通过视觉、听觉等产生心理应激。每次应激持续30min,隔天1次,连续2周。④30min运动组:第1周每天适应性游泳训练10min,然后每天无负重游泳1次,30min/次,共8周。⑤60min运动组:第1周每天适应性游泳训练30min,然后每天无负重游泳1次,60min/次,共8周。⑥心理应激+30min运动组。⑦心理应激+60min运动组。心理应激组大鼠末次应激24h后,其余各组大鼠在未次运动训练24h后取血和下丘脑以测定β-内啡肽和白细胞介素1含量。结果:①心理应激组血清白细胞介素1水平显著低于对照组[(0.317±0.104)μg/L,(0.520±0.150)μg/L]。下丘脑和血清中β-内啡肽的含量均明显高于对照组[(624.102±91.383)μg/g,(236.641±82.513)μg/g;(966.086±60.365)μg/L,(661.944±99.054)μg/L]。②心理应激+30min运动组和心理应激+60min运动组血清白细胞介素1的含量[(0.503±0.104)μg/L和(0.721±0.049)μg/L]均显著高于心理应激组,而下丘脑和血清中β-内啡肽的含量[(426.181±53.303)μg/g和(259.346±46.362)μg/g;(670.51±65.412)μg/L和(450.933±56.010)μg/L]显著低于心理应激组,其中以心理应激+60min运动组最为明显(P<0.01)。结论:在心理应激状态下中等负荷运动可明显抑制β-内啡肽含量升高,白细胞介素1含量下降。
AIM： To explore the effect of low and moderate exercise on content of β-endorphine （EP） and intedeukin-1 （IL-1） in rats under mental stress. METHODS： The experiment was conducted in the animal laboratory of Physical Education College, Yangzhou University from September to November 2003. Fifty-six healthy male SD rats ware selected and randomly divided into 7 groups： ① control group without any stress. ②etectdc stress group, which was stressed with serf-made electric stress cage with current of 1.0-2.0 rnA, voltage 30 V, cycle 100 seconds and 90 seconds as one interval, 30 minutes once, once every two days for 2 weeks. ③mental stress group： The rats ware allowed to watch the stress process of the stress group to form mental stress through vision and audition. The stress lasted for 30 minutes, once every two days for 2 weeks. ④ exercise for 30 minutes group： The rats swam for 10 minutes as adaptive training in the 1^at week, following by swimming without load once daily, 30 minutes each time for 8 weeks. ⑤exercise for 60 minutes group： The rats swam for 30 minutes as adaptive training in the 1^at week, following by swimming without load once daily, 60 minutes each time for 8 weeks. ⑥stress ＋ exercise for 30 minutes group. ⑦stress ＋ exercise for 60 minutes group. Twenty-four hours after the last stress of the mental stress group and the last training of other groups, the blood and hypothalamus of rats ware taken to determine the content of β-EP and IL-1. RESULTS： ①IL-1 level of the mental stress group was lower than the control group [（0.317±0.104）, （0.520±0.150） μg/L], and the content of β-EP in hypothalamus and serum was higher than the control group [（624.102±91.383） μg/g, （236.641±82.513）μg/g; （966.086±60.365） μg/L, （661.944±99.054） μg/L]. ②The contents of IL-1 of the stress ＋ exercise for 30 minutes group and stress ＋ exercise for 60 minutes group [（0.503±0.104）, （0.721 ±0.049）μg/L] ware obviouslyhigher than the mental stress group; but the contents of β-EP in hypothalamus and serum [（426.181 ± 53.303） μg/g, （259.346±46.362） μg/g; （670.51±65.412） μg/L, （450.933±56.010） μg/L] ware significantly lower than the mental stress group, especially the stress ＋ exercise for 60 minutes group （P 〈 0.01 ）. CONCLUSION： Low and moderate exercise can greatly inhibit the increase of β-EP and the decrease of IL-1 under mental stress.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第17期3354-3356,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
