摘要
目的:阐述钙离子与钙网织蛋白对心肌细胞的作用。资料来源:检索PubMed 1994-01/2005-12与钙离子和钙网织蛋白对心肌细胞作用的相关文章,检索词为“Ca2+,calreticulin,cardiomy-ocytes/cardiac myocyte,differentiation”,并限定语言种类为英语。资料选择:对所检索到的文献进行初筛,选择与钙离子和钙网织蛋白对心肌细胞作用的相关文章,筛除明显不符合原文的文章和重复性研究。资料提炼:共收集相关文章200篇,纳入42篇,排除158篇。资料综合:通过对内质网和肌质网上细胞内Ca2+释放通道(RyR和IP3R)的分子结构以及crt基因结构的研究表明:通道分子和钙网织蛋白共同参与调控体内Ca2+稳态,从而在心脏发育分化过程中发挥重要作用。结论:在心脏中细胞内游离Ca2+是一种关键的第二信使,细胞内Ca2+稳态的改变会显著影响许多心脏功能包括收缩舒张和心脏发育。细胞内Ca2+稳态的维持主要依赖内质网。在内质网中,钙离子主要与钙网织蛋白结合。crt基因敲除可损伤心脏发育而导致胚胎死亡。
OBJECTIVE： To elucidate the important role of calcium and calreticulin in cardiomyocyte DATA SOURCES： We searched PubMed database for articles about the role of calcium and calreticulin in the cardiomyocyte published in English from January 1994 to December 2005 with the key words of ＂Ca^2＋, calreticulin, cardiomyocytes/cardiac myocyte, differentiation＂. STUDY SELECTION： The retrieved data were selected primarily and articles about the important role of calcium and calreticulin in the cardiomyocyte were selected. Meanwhile, articles of repetitive research were excluded. DATA EXTRACTION ： Totally 200 articles were researched, 42 were included, and 158 were excluded DATA SYNTHESIS： Investigation on the molecular structure of the intracellular Ca^2＋ release channels （RyR and IP3R） on the endoplasmic reticulum and sarcoplasmic reticulum and structure of crt gene manifests that these channels co-participate in the regulation of calcium homeostasis with calreticulin, which plays a key role in cardiac development and differentiation. CONCLUSION ： Intracellular free Ca^2＋ is a critical second messenger in heart. Alterations in intracellular Ca^2＋ homeostasis have profound effects on cardiac functions such as contraction-relaxation and cardiac development. Intracellular Ca^2＋ homeostasis is maintained primarily via the endoplasmic reticulum, within which, Ca^2＋ is mainly bound to calreticulin. Knockout of crt gene is embryonically lethal due to impaired cardiac development.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2007年第3期592-595,600,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30370582,30440065,30572082)
北京市自然科学基金资助项目(7052059)~~
