摘要
利用AFLP分子标记技术对板栗短雄花序芽变及其母株进行了初步研究,72种引物组合共产生5129条清晰谱带,多态性位点53个,多态性2.05%,相似性系数0.9897,差异片段主要集中于300～900bp之间。研究表明短雄花序芽变和对照个体之间变异与遗传物质的改变相关,其中一些可能与雄花序变短的基因有关。
AFLP was used to compare the short catkins sport and its mother plant in chestnut （ Castanea mollissima Bl. ）. A total of 72 selective primer pairs amplified 5 129 clear bands, of those 53 were polymorphic loci. The polymorphic rate and Nei＇s association coefficient between them were 2. 05% and 0. 9897 respectively. The molecular weight range of the polymorphic fragments was mainly 300 -900 bp. The results indicated that there were some molecular-level differentiation between chestnut and its short catkins sport. Some of the specific segments had been considered as markers of short catkins in chestnut.
出处
《园艺学报》
CAS
CSCD
北大核心
2006年第6期1321-1324,共4页
Acta Horticulturae Sinica
基金
北京市自然科学基金资助项目(6052004)
北京市属市管高校人才强教计划资助项目
