摘要
采用四氯化碳（ＣＣｌ_４）制成原代培养大鼠肝细胞损伤模型，观察了绞股蓝总皂甙（ＧＰＳ）对肝细胞损伤的保护作用。结果表明，预先用ＧＰＳ处理肝细胞１ｈｒ，可明显减轻ＣＣｌ_４引起的肝细胞ＤＮＡ合成速率降低，减少肝细胞ＡＬＴ逸出，抑制培养上清液中ＭＤＡ含量的升高和ＳＯＤ／ＭＤＡ比值的缩小，并与ＧＰＳ浓度呈依赖关系。提示ＧＰＳ能减轻ＣＣＬ_４能减轻ＣＣｌ_４对离体培养的大鼠肝细胞损伤，保护肝细胞ＤＮＡ合成，其作用机理与抗脂质过氧化有关。
This in vitro study on the effect of Gypenosides(GPS) on carbon tetrachloride(CCl_4) induced injury in primary cultured rat hepatocytes showed that the decrease of their DNA synthesis rate and ALT leakage caused by CCl_4 were inhibited dose dependently when hepatocytes were pretreated with GPS,and the concentration of MDA was less and SOD/MDA more,in their medium,than that of injured control.It suggests the protection,which is associated with anti-lipid peroxidation,of GPS on CCl_4 induced hepatocellular injury in vitro.
关键词
绞股蓝总皂甙
四氯化碳
大鼠
肝细胞损伤
gypenosides
carbon tetrachloride
hepatocellular injury
lipid peroxidation
rat
