摘要
目的建立单核细胞增生李斯特菌(Listeria monocytogenes,LM)快速、敏感、特异的PCR诊断方法。方法采用聚合酶链式反应技术(PCR)特异性扩增单核细胞增生李斯特菌溶血素基因(hlyA),并评价该方法的特异性与敏感性。结果在706 bp处出现nuc基因的目的片断,只有单增李斯特菌的目的片段获得扩增,其他菌种扩增均呈阴性;该方法可以检测到3.3 ng/L的DNA。结论PCR方法比传统细菌检测方法更特异、快速、灵敏和简便,为食品中单增李斯特菌的快速检测提供了新的手段。
Objective To establish a rapid, sensitive and specific polymerase chain reaction （PCR） method for detection of Listeria rnonocytogenes （LM）. Methods Polymerase chain reaction （PCR） technique was used to amplifiy the hlyA gene of listeria monoey-togenes of food specifically , value the specificity and sensitivity of this method. Results The target gene nue was detected at 706bp, and only the target gene of Listexia monoeytogenes was amplified while the other bacteria was not detected; this method could also detect the DNA of 3.3ng/L. Conclusion The PCR method is more concise, rapid, sensitive and more specific than conventional culture method for detection of Listeria monoey-togenes in food. This method is useful for the investigation of listeria in food
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第1期60-61,共2页
Chinese Journal of Public Health
基金
吉林省卫生厅资助项目(085)
