摘要
采用PCR(聚合酶链式反应)的方法扩增并克隆了超慢生大豆根瘤菌(ESG,extra-slowlv-growing soyben rhizobia)2062菌株的16S rRNA的部分区段,然后进行核苷酸序列测定和分析。比较了测定的264个碱基序列与已经发表的其他相关根瘤菌序列的差异,并通过计算遗传距离进行聚类分析。结果表明,ESG与Bradyrhizobium japonicum、B. elkanii分别有5、11个碱基序列差异,系统发育关系较近;与Rhizobium、Azorhizobium、Agrobacterium属的种间有24～35个碱基序列差异,系统发育关系较远。16S rRNA部分序列测定既表明了ESG与其他根瘤菌在系统发育中的关系,又为这群ESG分类地位的确定提供了重要依据。
Bacteria strain 2062 is representative of ESG (extra-slowly-growing soybean rhizobia). The 260-bp segment of its 16S rDNA was amplified, cloned, and subsequently se-quenced. The data was compared with published sequences of relative bacteria. The distance was calculated and a phylogenetic tree was drawn based on the sequence data. The numbers of different nucleotides within the compared fragment were 5, 11, 24, 24, 29, 29, 30, 35 between ESG 2062 and Bradyrhizobium japonicum, B. elkanii, Azorhizobium caulinodans, Rhizobium fredii, R. galegae, R. leguminosarum, R. loti, and Agrobacterium tumefaciens, respectively. The sequence data indicated that ESG strain 2062 is phylogenetically related to Bradyrhizobium japonicum and B. elkanii.
出处
《微生物学报》
CAS
CSCD
北大核心
1996年第6期411-416,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目
