摘要
根据已公布的Hom eobox基因氨基酸序列保守区设计简并引物,利用PCR技术从内蒙古绒山羊血液基因组DNA中扩增Hom eobox基因家族成员。目的基因片段纯化后连接到pGEM-T载体上,经鉴定得到重组质粒。将110个重组质粒进行测序,测序结果通过与GenBank中序列比对分析,确定86条序列为Hom eobox基因,得到14个Hom eobox基因家族成员:Hoxa4,Hoxa5,Hoxa6,Hoxa7,Hoxb1,Hoxb2,Hoxb3,Hoxb6,Hoxb7,Hoxc6,Hoxd1,Hoxd3,Gbx1,Gbx2。每个基因片段由117个核苷酸组成,编码39个氨基酸。氨基酸序列非常保守,和其他物种的同源性非常高。
According to the conservative amino acid sequences of announced homeobox genes, degenerate primers were designed. The homeobox gene fragments of Inner Mongolian cashmere goat were amplified by PCR. The PCR products were purified and then cloned into pGEM -T vector. One hundred and ten 110 recombinant plasmids were sequenced. By blasting with database of GenBank,86 sequences were homeobox gene and classed into 14 kinds： Hoxa4, Hoxa5, Hoxa6, Hoxa7, Hoxbl, Hoxb2, Hoxb3, Hoxb6, Hoxb7, Hoxc6, Hoxdl, Hoxd3, Gbx1 and Gbx2. Every gene fragment was composed of 117 nucleotides which encoded a high conservative 39 amino acid peptide and the amino acid sequence had a high homology compared with other species.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2006年第6期919-922,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金地区重点项目(39963002)
