摘要
目的建立大鼠神经干细胞(NSCs)分离、培养方法,观察其生长、增殖和分化特点。方法利用无血清培养技术,从新生大鼠海马、室管膜下区分离NSCs,进行体外扩增培养、传代观察。采用荧光免疫细胞化学检测技术,观察鉴定NSCs及其分化结果。结果分离获取的细胞具有自我更新和增殖能力,原代及传代培养均可形成细胞克隆,克隆中的细胞巢蛋白(nestin)表达阳性,显微镜下观察见典型的干细胞特征,诱导后可分化神经元和星形胶质细胞。结论上法分离培养的细胞为具有自我更新和增殖能力的NSCs,可诱导分化为终末神经细胞。
Objective To isolate and identify neural stem cells （NSCs） from subventricular zone （SVZ） and hippocampus of neonatal rats, and to observe the characteristics of proliferation and differentiation of NSCs. Methods The serum-free medium was utilized to isolate and incubate NSCs from SVZ and hippocampus of neonatal rats. Fluorescence immunocytochemistry was applied to detect the antigen of nestin （neuroepithelial stem cell protein）, NF （neurofilament） and GFAP （glial fibrillary acidic protein）. Results The cells isolated from SVZ and hippocampus of neonatal rats possessed the ability of proliferation and self-renew. Nestin positive cells could be found in the cell clone sphere. Cells induced to differentiate could express antigen of NF and GFAP. Conclusion The NSCs were successfully isolate from SVZ and hippocampus of neonatal rats. The cells possess the ability of self-renew, proliferation, and differentiation.
出处
《中华神经医学杂志》
CAS
CSCD
2006年第11期1110-1112,共3页
Chinese Journal of Neuromedicine
基金
广东省自然科学基金(021862)
2004年科技计划项目(2060133)
