摘要
目的:观察脱矿脱细胞骨基质环孔隙度是否适合作为组织工程椎间盘纤维环细胞支架。方法:实验于2005-08/2006-01在解放军第三军医大学新桥医院中心实验室完成。①选用成年健康新西兰兔10只,按随机抽签法分为2组:实验组和对照组,每组5只。实验组取兔股骨近端骨制成环状,改进一般制备脱矿骨基质的方法,删除明胶化步骤,并进行脱细胞处理,使骨组织保留多孔结构,制成支架材料;对照组取兔股骨近端骨制成环状,不进行脱矿脱细胞处理。②两组均使用Zhang氏液体置换法测定材料的孔隙度。结果:新西兰兔10只均进入结果分析。实验组支架材料孔隙率为82.5%～91.1%,平均(87.8±2.8)%;对照组孔隙率为81.3%～88.4%,平均(85.5±2.2)%。两组标本孔隙度比较,差异不明显(P>0.05)。结论:经改进的方法制备的脱矿脱细胞骨基质环保持了骨的多孔结构,孔隙度指标达到作为支架材料的基本要求。
AIM： To explore whether the porosity of demineralized and deeellularized bone matrix annulus is suitable for annulus fibrosus disci intervertebralis tissue engineering scaffold. METHODS： The experiment was done in the Central Laboratory. of Xinqiao Hospital, the Third Military Medical University of Chinese PLA from August 2005 to January 2006.①Ten adult healthy New Zealand rabbits were selected and randomly divided into experimental group and control group, with 5 rabbits in each. In the experimental group, the bones were collected from the proximal ends of rabbits＇ femurs and manufactured into bone annuli. Then they were demineralized, decellularized and prepared into cell scaffolds by modified method without gelatin production so as to maintain the porous structure of bones. In the control group, the bones were also collected from the proximal ends of rabbits＇ femurs and manufactured into bone annuli. But they were not demineralized and decellularized. ②The porosities of the entire specimen were determined by liquid substitution developed by Zhang. RESULTS： Ten New Zealand rabbits all entered the result analysis. In the experimental group, the porosity of the scaffold ranged 82.5%-91.1%, mean （87.8±2.8）%. In the control group, the porosity of the scaffold was 81.3%-88.4%, mean （85.5±2.2）%. There was insignificant difference in the porosity between the two groups （P 〉 0.05）. CONCLUSION： The demineralized and decellularized bone matrix annulus manufactured by modified method can keep the porous structure like cancellous bone, and its porosity achieves the basic requirement for tissue engineering scaffold.
出处
《中国临床康复》
CSCD
北大核心
2006年第45期60-61,64,共3页
Chinese Journal of Clinical Rehabilitation
