摘要
本文报道了从网箱养殖的大口黑鲈（Ｍｉｃｒｏｐｔｅｒｕｓｓａｌｍｏｉｄｅｓ）皮肤溃烂病灶上分离到一株镰刀菌Ｆｕｌ－２，人工感染大口黑鲈鱼苗获得成功。Ｆｕｌ－２菌株经鉴定认为是镰状镰刀菌（Ｆｕｓａｒｉｕｍｆｕｓａｒｉｏｉｄｅｓ）。组织病理切片可见到感染的菌丝。Ｆｕｌ－２玉米碎低温培养物毒素粗提物中尚未发现有９种单端孢霉烯族毒素，但对豌豆发芽、兔子皮肤反应和三种鱼苗均显示出毒性，提示该培养物存在９种单端孢霉烯族之外的毒素。Ｆｕｌ－２对硫酸铜、氯化铜、食盐、小苏打等不敏感，对孔雀绿、亚甲基蓝、升汞等敏感。
This paper reports a fungi, Ful 2, isolated from the ulcerous skin lesion of Micropterus salmoides culturing in net cage. Artifical infecting Micropterus salmoides fry were successful, Ful 2 was preliminarily identified as Fusarium fusarioides. Ful 2 grows very well in PH 2.57 to 9.86. It tolerates higher salinity and can survive in media.with 13% NaCl. The infecting hypaes can be found from the histopathological sections. The crude extract of Ful 2 corn culture at low temperature was negative when tested for 9 kinds of trichothecenes toxin. The extract was toxic to pea seed germination, to rabbit skin response, to three kinds of fry, indicating the presence of a toxin other than 9 kinds of trichothecenes toaln. Ful 2 is highly sensitive to malachite green, methylene blue and mercury chloride etc, but isn't to cupric sulphate, cupric chloride, sodium chloide, sodium bicarbonate etc.
出处
《水生生物学报》
CAS
CSCD
北大核心
1996年第4期345-352,403,共8页
Acta Hydrobiologica Sinica
基金
广东省八五攻关项目
关键词
大口黑鲈
镰刀菌
水产微生物学
Micropterus salmoides, Ulceration, Fusarium, Toxicity, Trichothecene
