摘要
从不同生态环境中分离培养出20株光合细菌,进行5-氨基乙酰丙酸(5-ALA)的产量检测,筛选出一株分离自广州市云溪公园池塘底泥的菌株R5,其5-ALA产量最高,达4.92 mg/L.为了确定菌株R5的分类学地位,对该菌株的形态学、碳源利用情况等生理生化特性进行了研究,表明该菌株符合红假单胞菌属的生物学特征;以菌株R5基因组DNA为模板PCR扩增16S rDNA基因,对PCR产物进行了序列测定,将所测得的核酸序列与相关细菌的16S rRNA序列进行比对,并构建进化树进行系统发育分析,发现菌株R5与沼泽红假单胞菌株KUGB306(Rhodop-seudom onas palustrisKUGB306)形成一个类群,序列同源性为99%.故最后确定该高产5-ALA的菌株属于红假单胞菌属(Rhodopseudom onas).
5- aminolevulinic acid （5 -ALA） is a derivative of 5 -carbon amino acid and is found in most living organisms with photosynthetic bacteria having the highest extra - cellular production of 5 -ALA. In this study, the productions of 5 -ALA by 20 photosynthetic bacteria strains from different habitats were determined. Strain R5 isolated from the bottom mud of a pond in Yunxi park, Guangzhou, had the highest production of 5 - ALA （4. 92 mg/L ）. Its morphology and ability to utilize different carbon sources were consistent with the species of the genus Rhodopseudomonas. A phylogenetic tree constructed by the neighbour joining method based on the sequence of 16S rRNA fragment showed that strain R5 was clustered with Rhodopseudomonas palustrisKUGB306, with homology being 99%. Strain R5 with the highest production in the genus Rhodopseudomonas. of 5 -ALA should therefore be classified
出处
《暨南大学学报（自然科学与医学版）》
CAS
CSCD
北大核心
2006年第5期760-766,共7页
Journal of Jinan University(Natural Science & Medicine Edition)
