摘要
经改造的昆虫特异性神经毒素AaIT基因插入植物高效表达载体得到重组质粒pNGY-2。重组质粒中AaIT基因5＇端与烟草花叶病毒(TMV)的Ω序列3＇端融合,受两个串联的35S启动子控制,通过土壤农杆菌LBA4404介导转化烟草NC89,经NPTⅡ选择后再经GUS染色挑选出阳性再生植株,Southern blotting进一步证实了AaIT基因已经整合到烟草基因组中,对棉铃虫(Heliothis armigera)的抗虫实验表明,转基因烟草有显著的抗虫活性。
The recombinant plant expression vector pNGY-2 with designed and synthesized AaIT gene had been constructed. The AalT gene was fused downstream the sequence of TMV and inserted into expression vector under the control of two linked 35S promoters. The recombinant plasmid pNGY-2 was transferred into tobacco NC89 by agrobacterium-mediated leaf-disc gene transger system. The GUS activity analysis and Southern blotting of regenerated plants indicated that AalT gene had been introduced into tobacco genome. Insect bioassays showed that transgenic plants had notable insect-resistant activity.
出处
《生物工程学报》
CAS
CSCD
北大核心
1996年第2期113-118,共6页
Chinese Journal of Biotechnology
基金
Rockefeller fundation国际水稻生物技术项目资助
关键词
烟草
AaIT基因
转基因烟草
抗虫活性
AaIT gene, transgenic tobacco plants , insect-resistant activity
