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PDGF-BB促进人卵巢癌微血管内皮细胞增殖及PCNA、VEGF表达的实验研究 被引量：2

Response to stimulation of PDGF-BB in ovarian carcinoma-derived microvascular endothelial cells
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摘要 目的研究血小板源性生长因子BB(PDGF-BB)对人卵巢癌微血管内皮细胞(OdMEC)增殖、增殖细胞核抗原(PC- NA)和血管内皮生长因子(VEGF)表达的影响。方法体外分离培养OdMEC,施加不同浓度的PDGF-BB(0．5．10．20．50ng／ml),采用四甲基偶氮唑(MTT)法测定细胞生长曲线；免疫细胞化学及RT-PCR方法检测PCNA和VEGF表达的变化。结果PDGF-BB能促进OdMEC的增殖,呈剂量依赖性,以20ng／ml最为明显(P＜0．05)。在20ng／mI PDGF-BB刺激下,PCNA和VEGF的表达均高于时照组,差异有统计学意义(P＜0．05)。结论PDGF-BB促进OdMEC的增殖、PCNA和VEGF的表达。 Objective To study the influence of platelet derived growth factor-BB on the proliferation and VEGF expression in ovarian carcinoma-derived microvascular endothelial cells. Methods OdMECs were isolated and cultured from the carcinoma tissues. The cells were exposed to different concentration of PDGF-BB （0, 5, 10, 20, 50 ng/ml） and the growth curve was drawn by MTT and change of PCNA expression was observed. The expression of VEGF was detected by immunocytochemistry and RT- PCR. Results PDGF-BB could enhance OdMEC proliferation in close-dependent manner; and the effect of PDGF BB in 20ng/ml was more powerful than that in other concentrations. Stimulated by PDGF-BB in 20ng/ml, the expression of PDGF and VEGF was significantly increased than that in control groups （P〈0.01）. Conclusion PDGF-BB can promote the proliferation and expression of PCNA and VEGF in OdMEC.
作者 阎萍 杨鹰 黄健 韩静 连艳
机构地区 第三军医大学新桥医院妇产科 四川省绵阳市人民医院
出处 《重庆医学》 CAS CSCD 2006年第24期2255-2257,共3页 Chongqing medicine
关键词 卵巢癌 血管内皮细胞 细胞培养 血小板源性生长因子 ovarian carcinoma vascular endothelial cell cell culture platelet-derived growth factor
分类号 R737.31 [医药卫生—肿瘤] R730.21 [医药卫生—肿瘤]
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参考文献15

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二级参考文献13

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重庆医学
2006年 第24期

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