摘要
目的:复制大鼠佐剂性关节炎动物模型,并探讨蜂毒注射液在佐剂性关节炎中的疗效以及作用机制。方法:32只雄性SD大鼠,6只为正常对照组,26只大鼠用于佐剂性关节炎的模型制作,模型复制成功20只。将20只佐剂性关节炎大鼠随机分为3组,模型对照组6只、生理盐水治疗组6只、蜂毒治疗组8只。其中,模型对照组6只大鼠在治疗前进行处死,作为治疗前的水平。蜂毒治疗组大鼠每日皮下注射蜂毒注射液,连续14d。生理盐水治疗组大鼠注射相同体积的生理盐水。治疗前后记录关节周径、关节总体评分,治疗前后行X线检查,治疗结束时取血行TNF-α,IL-1β的检测及滑膜组织普通光镜检查。结果:与生理盐水治疗组的大鼠相比,蜂毒治疗组大鼠关节肿胀明显减轻,关节周径减少以及关节总体评分下降(P<0.05)。生理盐水治疗组大鼠X线检查骨质破坏,滑膜炎性细胞浸润比蜂毒治疗组大鼠明显。蜂毒治疗组大鼠血清中TNF-α,IL-1β的浓度比生理盐水治疗组的低(P<0.05;P<0.01)。结论:蜂毒对大鼠佐剂性关节炎有效,能减少滑膜中炎性细胞浸润,减少血管翳生成,降低血清中TNF-α及IL-1β水平,减轻骨质破坏。
Objective To investigate the anti-arthritic effect of bee venom in adjuvant induced arthritis （AIA） in rats. Methods Thirty-two male SD rats were enrolled in the experiment. Six were treated as negative controls and 20 AIA models were randomly divided into 3 groups： model controls （n = 6 ） , sodium chloride treatment group （n = 6 ） , and bee venom treatment group （n = 8 ）. The rats in the model control were killed before the treatment and the peripheral blood and synovium samples were collected for pre-treatment controls. The rats in the bee venom treatment group were injected hypodermically with bee venom for 14 days, while those in the sodium chloride treatment group were treated with the same volume of sodium chloride. During this period, the circumference of the affected joints and the total scores of the joints in all groups were measured every 2 days and X ray examinations were performed befor and after the treatment. At the end of the treatment, all the rats were killed and their peripheral blood and synovium samples were collected for measurements of tumor necrosis factor TNF-α and interleukin IL-1β and histological studies, respectively. Results Compared with the sodium chloride group, the rats in the bee venom treatment group were less swollen in joints and circumference of joints and lower joint scores decreased （ P 〈 0.05 ）. At the same time, the bone erosion and the infiltration of inflammatory cells in the synovium were also significantly reduced in the bee venom treatment group. In addition, the serum concentrations of TNF-α and IL-1β were significantly lower in rats of the bee venom treatment group than those of the sodium chloride group （P 〈 0.05 and P 〈 0.01, respectively ）. Conclusion Bee venom is effective in treating AIA by reducing synovitis, downregulating the serum concentrations of cytokine TNF-α and IL-1β and alleviating the bone erosion.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第6期948-951,共4页
Journal of Central South University ：Medical Science
