摘要
小鼠不是HBV的自然宿主,不支持HBV的感染、复制,需通过一定的手段才能使其感染HBV。转基因小鼠是目前应用最多的研究HBV的动物模型,但因不能用于病毒进入以及免疫清除机制等方面的研究而限制了其应用。通过腺病毒载体介导或通过注射质粒等方法也可导致HBV在小鼠体内短暂复制,因而可作为研究HBV急性感染的模型。将人肝细胞移植到免疫缺陷小鼠体内形成嵌合小鼠来研究HBV是最近研究的热点,嵌合模型更加接近自然感染,可以用于病毒的早期进入、复制以及抗病毒治疗的研究,缺点是应用了免疫缺陷小鼠,限制了其在病毒免疫方面的研究,因此目前尚需研发更加完善的HBV动物模型。
Mouse is not the natural host of HBV, so it can not allow HBV＇s infection and replication unless the viral genome is transfected into mouse hepatocytes by different means. Transgenic mouse is the major model for investigators to elucidate the mechanisms of HBV replication, but it can not allow the study of viral entry and the investigation of mechanisms of viral clearance. HBV can transiently replicate in mice by some other means, such as recombinant adenoviral vectors or by hydrodynamic injection of naked DNA in mice, which provided the models for acute infection of HBV. The humanized-mouse models, which are based on transplantation of human hepatocytes into immunodeficient mice, at- tracted more and more scientists＇ attention. The models allow us to investigate the mechanisms of viral entry and replication and to study the antiviral treatments. But the mice used are immunodeficient, so we have to develop more consummate models.
出处
《国际流行病学传染病学杂志》
CAS
2006年第6期392-395,共4页
International Journal of Epidemiology and Infectious Disease
基金
国家973计划(2004CB518807)
