摘要
目的：从阿片受体角度观察电针（EA）对佐剂性关节炎（AA）大鼠产生镇痛的外周机制。方法：将32只SD大鼠随机分为正常组、模型组、EA组和单针组。于实验第1天在模型组、EA组大鼠右后足垫部向踝关节方向注入弗氏（Freund’s）完全佐剂0．1mL造模。以“悬钟”“昆仑”为EA穴位，刺激参数为2～4V，20～100HZ，每天1次，每次20min，连续6d。以原位杂交等为检测方法，在EA治疗后观测大鼠痛阈、炎症局部8阿片受体（DOR）mRNA的表达。结果：①EA可明显提高炎症痛大鼠的痛阈，EA组与模型组比较有显著性差异（P〈0．01），而与正常组无显著性差异（P〉0．05）；②EA组炎症局部DOR mRNA的表达明显高于模型组（P〈0．01）。结论：EA对AA大鼠有明显镇痛效应，且镇痛效应的产生与大鼠炎症局部的DORmRNA表达增强有关。
Objective： To observe the effect of electroacupuncture （EA） on delta （6）-opioid receptor gene expression in the inflammatory tissue in adjuvant arthritis rats so as to study the underlying peripheral mechanism of EA analgesia. Methods： Thirty-two SD rats were randomized into control,modeI,EA （model＋ EA） and normal＋ EA （normal animals） groups with 8 cases in each group. EA （2-4 V, 20- 100 Hz） was applied to ＂Xuanzhong＂ （GB 39） and ＂Kunlun＂ （BL 60） for 20 min, once daily, and 6 sessions all together. Arthritis model was established by injection of Freund＇s complete adjuvant （0. 1 mL） in the rat＇s right hindpaw. The latency of radiation-heat irradiation induced leg withdrawal was considered as the pain threshold. At the end of experiments, the animals anesthetized with 3% phenobarbital （30 mg/kg） were transcardiacally perfused with 4% paraformaldehyde and 0. 1% diethylpyrocarbonate, followed by taking the local focus tissue. After routine treatment, the tissue samples were embedded with paraffin, cut into sections （6 μm） and processed with in situ hybridization histochemistry for observing the expression of delta opioid receptor mRNA. Results： （1） Compared with control group, the pain threshold of model group declined significantly （ P〈0.01 ）, while comparison between EA and model groups, and between normal ＋ EA and model groups showed that the pain thresholds of two EA groups were significantly higher （ P〈0.01 ）, and the threshold of normal ＋ EA group was also significantly higher than that of EA group （P〈0.01）. No significant difference was found between control and EA groups in the pain threshold （P〉0.05）, indicating that EA could raise the pain threshold in both arthritis and normal rats. （2） Compared with model group, the number of 6 opioid receptor mRNA expression-positive cells of EA group was remarkably more （P〈0.01）, showing marked upregulation of δ opioid receptor mRNA expression in the focal tissue after EA. Conclusion ： EA has a definite analgesic effect in arthritis rats, which may be related to its effect in upregulating the expression of δ opioid receptor mRNA expression.
出处
《针刺研究》
CAS
CSCD
2006年第6期333-336,共4页
Acupuncture Research
基金
广东省中医药管理局科研基金资助项目(97246)
