摘要
目的检测15-脂氧合酶-1(15-LOX-1)mRNA及蛋白在胃癌及癌旁正常组织中的表达,并探讨15-LOX-1表达与胃癌临床病理因素的关系。方法采用RT-PCR、westernblot和免疫组织化学方法检测胃癌组织及相匹配的癌旁正常组织中15-LOX-1mR-NA和蛋白的表达。结果15-LOX-1mRNA及蛋白在胃癌组织中的表达均显著低于癌旁正常组织(P<0.05)。15-LOX-1蛋白表达水平与胃癌中肿瘤大小、淋巴结转移和TNM分期呈明显的负相关(P<0.05)。结论15-LOX-1蛋白可能对胃癌的发生发展有一定抑制作用。检测胃癌中15-LOX-1的表达情况对评估患者的预后可能具有意义。
Objective To detect the expression of 15-1ipoxygenase-1 （15-LOX-1） mRNA and protein in gastric carcinoma and adjacent normal tissue, and to investigate the relationship between the expression of 15-LOX-1 and clinical pathological characteristics. Methods The 15-LOX-1 mRNA and protein level were detected in pairs of gastric carcinoma and adjacent normal tissue by reverse transcription polymerase chain reaction（RT-PCR）,immunohistocbemistry and western blot methods. Results The expression of 15-LOX-1 mRNA and protein in gastric carcinoma was lower than those in the adjacent norreal tissue significantly（ P 〈 0.05）. There was significantly negative correlation between 15-LOX-1 protein expression and clinical pathological characters such as tumor size,lymph node metastasis and TNM stage（ P 〈 0.05）. Conclusion The 15- LOX-1 protein may restrain the genesis and progress of gastric carcinoma. The assay of 15-LOX-1 expression in gastric carcinoma may be helpful to evaluate the prognosis of the patients with gastric carcinoma.
出处
《胃肠病学和肝病学杂志》
CAS
2006年第6期576-580,共5页
Chinese Journal of Gastroenterology and Hepatology
基金
福建省科技人才创新基金资助项目(No.2002J061)
