摘要
目的探讨二硫代氨基甲酸吡咯烷(PDTC)对实验性脑出血血肿周围脑组织含水量和水通道蛋白9(AQP9)表达的影响。方法将128只SD大鼠随机分成正常组、对照组、脑出血组及PDTC组,建立脑出血模型;制模2 h予PDTC组腹腔注射PDTC;通过干湿法和SABC法检测各组大鼠各时点脑组织含水量、AQP9表达。结果(1)脑出血组脑组织含水量出血侧在制模后4 h开始升高,72 h达高峰,持续到120 h后下降;出血对侧在4 h也升高,48 h达高峰;脑组织含水量较对照组明显增加,出血侧更明显(均P<0.01)。(2)脑出血组AQP9表达制模后4 h开始升高,72 h表达最强,120 h降低,较对照组明显增加,出血侧较出血对侧增加更明显(均P<0.01)。(3)PDTC组脑组织含水量和AQP9表达在制模后24 h开始各时间点较脑出血组明显降低(均P<0.01)。结论脑出血后脑组织含水量和AQP9表达均呈平行增加,PDTC干预对出血后脑组织含水量和AQP9表达有平行抑制作用,提示AQP9参与了脑出血后脑水肿的形成。
Objective To investigate the effects of pyrrolidine dithiecarbamate （PDTC） on aquaporin-9 （AQP9） expression and brain edema around the hematoma in experimental intracerebral hemorrhage （ICH）. Methods 128 SD rats were randomly divided into four groups： normal group, control group, ICH group and PDTC group. According to Rosenberg＇s methods, the models of experimental ICH were established. The rats in PDTC group were received PDTC intraperitoneally 2 h later. The brain weight column （BWC） at different time point was calculated by gray-wet method, and the expression of AQP9 was examined by immunohistochemistry. Results The BWC and expression of AQP9 in hematoma side of ICH begin to increase 4 h after ICH, peaked at 72 h, and decreased 120 h later. The BWC in opposite side of hematoma also increased 4 h after ICH and peaked at 72 h. Compared with control group, the BWC and expression of AQP9 in ICH group were higher, especially in hematoma side of brain （all P 〈 0. 01 ）. Compared with ICH group, the BWC and expression of AQP9 in PDTC group significantly decreased 24 h after ICH （ all P 〈 0. 01 ）. Condusions BWC and the expression AQP9 increase at the same time after ICH and may be inhibited by intervention of PDTC. AQP9 is involved in the brain edema after ICH.
出处
《临床神经病学杂志》
CAS
北大核心
2006年第6期444-446,共3页
Journal of Clinical Neurology
