摘要
用限制性酶切片段长度多态性分析对沈阳地区７０例血清经巢式聚合酶链反应扩增ＨＢＶＤＮＡ阳性的乙型肝炎患者的Ｃ基因前Ｃ区１８９６位点进行分析。结果：（１）急性乙型肝炎前Ｃ区变异检出率为４％（１／２０），慢性迁延肝炎为２５％（５／２０），慢性活动性肝炎为３８％（５／１３），肝硬化为３３％（４／１２）；（２）ＡＬＴ升高组中，ＨＢｅＡｇ及抗ＨＢｅ均阴性患者和ＨＢｅＡｇ阴性，抗ＨＢｅ阳性患者ＨＢＶＤＮＡ前Ｃ区变异检出率分别为５７％和５０％，明显高于ＡＬＴ正常组（１７％和０％）。上述结果说明ＨＢＶ感染时间长短和肝功反复活动与ＨＢＶＤＮＡ前Ｃ区变异有关。
Sera from 70 patients with HBV infection were detected for HBV DNA by nested PCR. G to A mutants at 1896 sites on HBV preC region were tested positive by restriction fragment length polymorphism analysis. The findings were: (1) The positive rate of mutant on HBV preC region was 4%(1/25) in acute hepatitis, 25% (5/20) in chronic persistent hepatitis, 38% (5/13) in chronic active hepa titis, and 33% (4/12) in cirrhosis; (2) The positive rate of mutant on HBV preC region in patients with higher serum ALT level was 57% im HBeAg(-)/ant HBe(-) group and 50% in HBeAg(-)/anti HBe(+) group, and these were significantly higher than that in the patients with normal serum ALT level. Therefore, the results suggested that for the escape from host immunity, hepatitis B virus constantly made itself HBV DNA variant which had to be involved in the mutant on HBV preC region of patients with chronic hepatitis.
出处
《中国医科大学学报》
CAS
CSCD
1996年第5期505-507,510,共4页
Journal of China Medical University
基金
国家自然科学基金
