摘要
目的:研究外源核苷酸对脂多糖(LPS)刺激小鼠的保护作用,并探讨其可能机制。方法:采用刚断奶的昆明种仔鼠40只,按体重随机分成五组:对照组、核苷酸(NT)组(4h、18h)、无核苷酸(NF)组(4h、18h),对照组和NF组饲喂无核苷酸半纯合基础日粮;NT组饲喂含0.25%核苷酸混合物的日粮,在实验的D15,NT组和NF组灌胃LPS,对照组灌胃同等剂量的无菌生理盐水,在灌胃后的4h、18h,收集血清,无菌取肝、小肠、腹腔巨噬细胞待测。结果:日粮中添加核苷酸能显著升高LPS刺激小鼠肝Na+K+-ATP酶、小肠超氧化物歧化酶(SOD)活性、血清总抗氧化能力(T-AOC)和腹腔巨噬细胞分泌的抗炎性细胞因子白介素10(IL-10)的水平,降低小肠丙二醛(MDA)含量、血清谷丙转氨酶(AIL)、小肠髓过氧化物酶(MPO)活性和腹腔巨噬细胞分泌的炎性细胞因子白介素1(IL-1)的水平。结论:小鼠在受到LPS刺激时,在无核苷酸日粮中添加核苷酸有助于维持机体氧化/抗氧化、炎症/抗炎症平衡,保护机体免受损伤。
Objective： To study the protective effects of exogenous nucleotides on lipopolysaccharide （LPS） immunnostimulation and its mechanism. Method： Forty healthy Kunming mice were randomly divided into five groups： control group, nucleotides （NT） groups （4h, 18h）, nucleotides free （NF） groups （4h, 18h）. Control group and NF groups were fed with nucleotide-free diet. NT groups were fed with nucleotide-supplemented diet （0.25% nucleotides）. On D 15, mice were lavaged with physiological saline （control） or LPS, and were killed 4 or 18 h later. Serum, liver, small intestine, and peritoneal macrophage were sampled in germfree state. Results： Hepatic Na^＋K^＋-ATPase, intestinal superoxide dismutase （SOD）, serum total anti-oxidation ability, peritoneal macrophage-produced interleukin 10 （IL-10） were increased, and intestinal malonaldehyde （MDA）, serum alanine aminotransferase （ALT）, intestinal myeloperoxidase （MPO）, peritoneal macrophage-produced interleukin 1 （IL-1） were decreased with nucleotides supplement. Conclusion： Exogenous nucleotides can help to maintain oxidation-antioxidation and inflammation-antiinflammation balance, and protect mice from injury under LPS immunostimulation.
出处
《营养学报》
CAS
CSCD
北大核心
2006年第6期468-471,共4页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.30571347)
关键词
核苷酸
脂多糖(LPS)
氧化
炎症
nucleotides
lipopolysaccharide （LPS）
oxidation
inflammation
