摘要
采用戊二酸酐法合成展青霉素-半戊二酸(PAT-HG),通过活泼酯法将PAT-HG偶联到牛血清白蛋白,制备了展青霉素免疫抗原(PAT-HG-BSA),免疫3只BALB/c小鼠,经4次免疫后,1号小鼠血清抗展青霉素抗体效价达1∶1600,且小鼠抗展青霉素多克隆抗体与展青霉素检测抗原的结合能被展青霉素阻断。
The hemiglutarate-patulin （PAT-HG） was prepared by esterification with glutarate, then, it was conjugated to bovine serum albumen（BSA） to form immunogen （PAT-HG-BSA） by ester method. The titer of anti-PAT antibodies in one BLAB/c miee serum, immunized by PAT- HGrBSA, was about 1 ： 1600, in which antibodies binding to PAT-MOVA was inhibited by free patulin.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第6期13-17,53,共6页
Journal of Food Science and Biotechnology
基金
国家"十五"重大科技专项课题(国科农社函[2002]130号)
关键词
展青霉素
人工抗原
多克隆抗体
patulin
artificial antigen
polyclonal antibody
