摘要
研究了用重组孕激素受体基因酵母测定饮用水中内分泌干扰物的方法,并利用该种方法检测了南方某水厂不同处理工艺过程水样对孕激素受体活性的抑制水平.结果表明,重组孕激素受体基因酵母能够与孕激素专一性的结合,诱导产生明显的剂量-效应关系,EC50值为0.5 nmol/L,具有较高灵敏度;环境内分泌干扰物五氯酚和壬基酚具有孕激素受体抑制活性,其IC50值分别为2.4μmol/L和3.7μmol/L;重组孕激素受体基因/报道基因的酵母技术是一种筛选和定量分析具有孕激素受体抑制活性的内分泌干扰物的快速、有效方法.结合固相萃取的前处理技术,重组孕激素受体基因酵母对水厂不同处理工艺水样检测出明显的孕激素受体抑制活性,抑制率均大于58%,表明重组孕激素受体基因酵母检测技术能够快速监测和鉴别水样中具有抑制孕激素受体活性的物质.
A bioassay method using recombinant human progesterone receptor （hPR） gene yeast to evaluate the environmental endocrine disrupter effects was introduced. This method was used to determine the inhibition of hPR activity in pollutants emitted from a water works in the south China. The result showed that the recombinant gene yeast had steroid specificity and dose respond curve for progesterone with ECs0 value of 0.5 nmol/L; The estrogenic chemicals pentachlorophenol and p-nonylphenol inhibited the activityof hPR in the yeast which had IC50 values of 2.4μmol/L and 3.7 μmol/L, respectively. The results indicate that the recombinant progesterone gene yeast assay technique is an efficient and rapid method for screening and quantitatively analyzing the endocrine disrupters which can be the inhibitor of hPR activity, Combined with the solid-phase extraction （SPE） procedure, the yeast assay showed an obvious inhibition of hPR activity for water works sample and the inhibition rates were more than 58 %. Therefore, it is reliable to use the recombinant hPR gene yeast for assessing the environmental endocrine disrupters.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2006年第12期2463-2466,共4页
Environmental Science
基金
国家自然科学基金项目(50378089)
国家高技术研究发展计划(863)项目(2002AA601120)
