摘要
目的探讨乙型肝炎病毒(HBV)特殊血清学表现模式。方法A试剂检测到的乙肝特殊模式标本用B、C两种试剂重检;对HBeAg阳性、HBsAg阴性标本用倍比稀释和二步法重做HBsAg;以聚合酶链反应(PCR)检测乙肝特殊模式的HBVDNA。结果A试剂检测到的乙肝特殊模式10种,145例;分为“12”同时阳性和“3”阳性、“1”阴性两个模式组;用B、C两种试剂复检的结果同A试剂相比存在较大差异;用倍比稀释和二步法检测“35”模式的HBsAg,阳性率分别提高到75.0%和80.0%,与其HBVDNA的阳性率(74.0%)相一致。结论不同厂家试剂对乙肝特殊血清学模式的检测结果存在差异。“35”模式标本一步法试剂检测HBsAg漏检率高,用倍比稀释或二步法重检可提高HBsAg的阳性率,应结合HBVDNA的检测结果作出综合判断。
Objective To study the correlation between special serological patterns in hepatitis B virus （HBV） infection and serum HBV-DNA content. Methods Reagent B and C were used to re-test the positive specimens determined by reagent A for special HBV serological patterns. For the cases of HBsAg- HBeAg^＋ samples were tested by continuous dilution and two-step method. Serum HBV-DNA content was measured by pelymerase chain reaction （PCR）. Results Ten modes of special HBV serological distribution were found by using reagent A and classified as two groups： the＂1^＋ 2^＋＂ 24 group and ＂1ˉ 3^＋ ＂ group. The results obtained by reagent B and C were different from A. Compared to one-step method, the positive rate of ＂35＂ mode detected by continuous dilution and two-step method was increased by 75.0% and 80.0%, respectively. Furthermore, the results of continuous dilution and two-step method were more likely to be consistent with the results of PCR （74.0%）. Conclusion The detected result of special serological patterns in HBV are different according to different source of reagents. The false negative of HBsAg is high in ＂35＂ mode by one-step method, while this problem can be resolved by continuous dilution and two-step method. We should combine routine serological results with HBV DNA content measurement to determine true virus infection status.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2006年第4期405-406,共2页
Chinese Journal of Experimental and Clinical Virology
