摘要
目的了解安徽省部分医院乙型肝炎病毒(HBV)基因型的分布及其与临床的相关性。方法选择安徽部分医院HBVDNA阳性的HBV感染者196例,其中急性黄疸型肝炎3例,慢性乙型肝炎107例,肝炎后肝硬化59例,原发性肝癌6例,慢性重型肝炎20例,亚急性重型肝炎1例,采用Caliper-1000微流芯片分析仪分析检测HBVDNA和基因型。结果196份HBVDNA阳性血清标本中,B基因型126例(64·3%),C基因型59例(30·1%),BC混合型11例(5·6%),未发现其他基因型。江淮之间以B基因型占优势,淮河以北以C基因型占优势,分别为68·8%和66·7%;HBVB基因型感染的临床疾病谱以慢性乙型肝炎为主,占62·7%(P<0·01),C基因型感染者以肝硬化为主,占44·1%(P<0·01),差异有显著性;检测出的HBV基因型与病毒载量、HBeAg阳性表达差异均无显著性;肝硬化患者HBVC基因型的HBeAg阳性表达(68·4%)明显高于B基因型(31·6%)和BC混合型(0)。结论安徽省部分医院HBV基因型存在B、C和BC混合型,B型为该地区的优势基因型。HBVB基因型感染的临床疾病谱以慢性乙型肝炎为主,而HBVC基因型感染以肝硬化为主。
Objective To investigate the distribution of hepatitis B virus genotypes in several hospitals of Anhui province and its clinical significance. Methods Serum samples from 196 HBV DNA positive patients with HBV infection, including 3 acute icteric hepatitis（AIH）, 107 chronic hepatitis B（CHB） ,59 liver cirrhosis（LC） ,6 hepatocellular carcinoma （HCC）,20 chronic severe hepatitis （CSH）,and 1 subacute severe hepatitis （SSH）, were collected and tested for HBV genotypes by Caplier-1000 Microchip electrophoresis. Results Of the 196 patiens, 126 （64. 3% ） were genotype B and 59（30.1% ） were genotype C, the rest of them 11 （5.6%） were genotype BC combination. Genotype B was preponderant between changjiang and huaihe river, and was more prevalent than genotype C in the CHB and LC （ P 〈 0.05 ）. The value of HBV DNA and the rate of e-Ag positive was no significant among the detected genotypes, while significantly higher in genotype C than in genotype B （ P 〈 0. 05 ） in the patients of the LC group. Conclusion Genotype B, C and BC combination exist in several hospitals of Anhui province, and genotype B is the major genotype in this area. The outcomes of HBV genotype B infection is related to CHB, and genotype C is correlated with LC.
出处
《安徽医科大学学报》
CAS
北大核心
2006年第6期676-679,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省临床医学重点学科技术进步专项课题(编号:2004Z017)
安徽省"十五"科技攻关"传染病综合防治研究"专项基金(编号:040130325)
安徽省高等学校省级自然科学基金(编号:2006KJ372B)
