摘要
目的：了解中国汉族人群中自细胞介素10(IL-10)启动子区基因多态性的等位基因频率。方法：应用聚合酶链-限制性片段长度多态性分析(PCR-RFLP)技术,对131例健康中国汉族受检样本进行IL-10—592、-819、-1082三个位点的基因型检测。结果：IL-10—592位点A/A、C/A、C/C基因型频率分别为42．7%、36．6%、20．7%,IL-10—819位点T/T、T/C、C/C基因型频率分别为42．7%、36．6%、20．7%；其相关等位基因频率与意大利高加索人及英国曼彻斯特人相比有显著性差异,但与韩国人之间的差异无统计学意义。结论：不同国家人群间存在IL-10启动子区基因多态性的差异。
Objective： To observe the allele frequencies of polymorphisms of interleukin 10 （IL-10） promoter in Chinese Han population. Methods： PCR-restriction fragment length polymorphisms assay （PCR-RFLP） method was used to detect the genotypes distribution of IL -10 gene promoter 592, 819,-1082 positions of 131 healthy Chinese Han subjects. Results： The frequencies of A/A, C/A, C/C genotypes at IL-10 gene promoter-592 position were 42. 7% ,36.6% and 20.7%, respectively; those of T/T, T/C, C/C genotypes at IL-10 gene promoter -819 position were 42.7%, 36.6% and 20.7%, respectively; and those of G/G, G/A, A/A genotypes at IL-10 gene promoter-1082 position were 0%, 8.4% and 91.6% , respectively. The allele frequencies of polymorphisms of IL-10 were significantly different from those of the Italian and UK populations, but were similar to those of korea population. Conclusion： It indicates that there is heterogeneity in the frequencies of polymorphisms of interleukin-10 promoter among different populations.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2006年第6期686-689,共4页
Chinese Journal of Anatomy
