摘要
目的探察Shh对E13-E15小鼠胚胎中视觉传导通路发育的影响。方法E13至E15小鼠胚胎的眼至视束部分制备成脑厚片,置于含10%的小牛血清的DMEM/F12的培养液中,在37℃恒温滚动培养箱中培养5h。实验组中将Shh抗体加入培养液中。培养结束后,将脑厚片以4%多聚甲醛固定,将DiI颗粒置于视盘。7d后,在手术显微镜下,暴露被标记的视神经纤维,在激光扫描共聚焦纤维镜下观察。结果用Shh抗体阻抑Shh的功能,可引起E13跨越中线的视神经纤维减少以及E15投射至同侧的视束的神经纤维的增多。结论在视交叉形成的早期,Shh引导视神经纤维跨越中线。在视交叉形成的后期,Shh引导视神经转弯。
Objective To understand the function of Sonic hedgehog in chiasm development in mouse embryos of embryonic day 13 （E13） to E15. Methods Brain slices of E13-E15 mouse embryos containing the optic pathway from the eyes to the optic tract were prepared and cultured in DMEM/F12 in the presence of 10% fetal bovine serum at 37 ℃ in a rolling incubator for 5 h. The antibody to Shh was added into the culture medium of the slices in the treatment group, while no additional chemical or only normal mouse IgG was added in the control groups. After culture, the brain slices were fixed and a DiI granule was inserted into the optic disc in one eye. Seven days later, the tissue overlying the chiasm was removed to expose the DiI-labeled chiasm for observation under confocal microscope, and the images were analyzed by METAMORPH software. Results Shh antibody treatment produced a reduction of crossing of the earliest retinal axons at the midline of E 13 chiasm, and the uncrossed axons were also influenced by Shh antibody at E15. Conclusion Shh executes a transient but important function in axon decussation in the early stage of mouse optic chiasm development and signals axon turning in the later stage.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2006年第12期1679-1684,共6页
Journal of Southern Medical University
基金
Supported by Research Grant Council of Hong Kong(CUHK 4417/03M)
关键词
SHH
视交叉
发育
Sonic hedgehog
chiasm
development
