摘要
目的DRE-PCR DNA指纹分型方法的建立及其在分枝杆菌基因分型中的应用。方法收集结核病患者痰标本，分离培养获得分枝杆菌，进行表型鉴定及耐药性检测；提取基因组DNA，在建立DRE-PCR DNA指纹分型方法的基础上对分枝杆菌进行DNA指纹图谱分析。结果DRE-PCR指纹图谱分析将80株分枝杆菌分为13种菌型，其中A型菌26株（32．5％），B型菌14株（17．5％），C型菌16株（20．0％），其余各型均〈7．5％。分组统计显示，3种主要的DRE-PCR DNA指纹在40～60岁组的分布偏高（P=0．019），而与患者的性别、职业、长期居住地、结核接触史、痰涂片、耐药性等无关。结论DRE-PCR DNA指纹分型方法是一种简单、快速的分枝杆菌的分型方法；3种主要类型的结核分枝杆菌在唐山地区流行。
To establish the double repetitive element-PCR （DRE-PCR） molecular typing method to be used in the genotyping of Mycobacterium tuberculosis （MTB）, 80 sputum specimens of patients with pulmonary tuberculosis were collected and cultivated at 37℃ to obtain the clinical isolates of MTB. These isolates were identified by means of the phenotyple method. And their chromosomal DNA was extracted. The DNA-fingerprinting analysis of MTB was performed on the basis of the established method of DRE-PCR. As analyzed with this method, the 80 MTB isolates were finally classified into 13 genotypes, in which most of them belonged to A （32.5%）, B （17.5%） and C （20.0%） genotypes and the other 10 genotypes appeared to be scattered. There was a significant difference among different age groups in the DRE-PCR fingerprinting. This result indicates that the DRE-PCR based DNA fingerprinting analysis is proved to be a fast and simple molecular typing method used for the genotyping, and there exists three main genotypes in the epidemic strains of Mycobacterium tuberculosis, in Tangshan city.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第12期1143-1146,共4页
Chinese Journal of Zoonoses
基金
卫生部科技攻关项目子课题
No.2003BA712A11-24
