摘要
目的:研究β-干扰素(INF-β)对肝癌根治性切除后复发和生长的抑制作用。方法:将人肝癌组织植入25只BALB/c裸鼠肝脏,建立人肝癌原位移植瘤模型。第10 d完整切除荷瘤后,随机分为3组:A组对照组(生理盐水皮下注射);B组小剂量实验组(INF-β3×105U/d皮下注射);C组大剂量实验组(INF-β6×105U/d皮下注射)。连续注射35 d后处死裸鼠,观察肿瘤复发情况,测量肿瘤大小和体积;免疫组织化学法检测肿瘤增殖核抗原(K i-67)、诱导型一氧化氮合酶(iNOS)和半胱氨酸天冬氨酸蛋白酶-3(caspase-3)表达。结果:A组肿瘤复发率为100%,B组为87.5%,C组为77.8%,3组比较无显著差异(P>0.05)。B组和C组抑瘤率分别为73.6%和94.3%,与A组比较差异显著(P<0.05)。B组和C组肿瘤组织iNOS、K i-67的表达明显低于A组,而caspase3的表达明显高于A组(P<0.05)。结论:β-干扰素可有效抑制肝癌切除术后复发肿瘤的生长。大剂量β-干扰素较小剂量对肝癌的生长抑制作用更强,其机制可能与肿瘤组织内iNOS、K i-67和caspase3的表达有关。
AIM： To study the effect of interferon - β（ INF - β） on recurrence and growth of intrahepatic HCC after radical resection in nude mice. METHODS： Human HCC cells were implanted into the livers of 25 BALB/c nu/nu nude mice. All the mice were received radical resection of HCC at day 10, and divided into three groups randomly In group B and C, INF -β was injected subcutaneously at different dosage for each mouse respectively. In group A, nude mice were given saline at the same volume instead of INF -β. At 35 days after treatment, all mice were sacrificed, liver specimens were harvested, the size and volume of recurrent tumor were also measured to calculate the tumor inhibition rate of INF -β. Expression of iNOS, Ki - 67 and caspase - 3 in recurrent tumor were detected by immunohistochemistry. RESULTS ： No difference in HCC recurrent rate among group A, B and C was observed （P 〉 0. 05 ）. Tumor volumes was diminished in group B and C as compared with that in group A （ P 〈 0. 05 ）. Expression of iNOS and Ki - 67 in group B and C was decreased as compared with that in group A （ P 〈 0. 05）. Expression of caspase - 3 in IFN -β treated group was increased as compared with that in control group （ P 〈 0. 05 ）. CONCLUSION ： IFN -β has an inhibitory effect on growth of intrahepatic HCC after radical resection in nude mice. The inhibitory effect of IFN -β is associated with the dose used, probably related to the changes of iNOS, Ki - 67 and caspase - 3 expression in tumor tissue.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第12期2385-2389,共5页
Chinese Journal of Pathophysiology
基金
瑞士雪兰诺公司科研部横向实验基金资助项目
