摘要
结合提取基因组DNA的CTAB法(hexadecyltrimethylammoniumbromide,十六烷基三甲基溴化铵)和用于提取海藻基因组DNA的LiCl法,设计了针对产生较多荚膜多糖细菌的DNA提取方法—LiCl沉淀法。通过电泳、分光光度计及16SrRNA基因扩增检测,证明LiCl沉淀法可以有效地提取产荚膜细菌基因组DNA,其片断大小约为23kb,不需进一步纯化即可用于后续分子生物学实验。
Bacteria rich in capsule are widely applied in many fields and it is important to investigate their gene resources. But capsules, especially capsule polysaccharides, will disturb DNA extracting largely, thus, LiCI precipitating method was introduced in this paper as a novel method and was examined by electrophoresis, spectrophotometer and amplification of 16S rRNA gene. Results showed that it could extract genomic DNA about 23 kb effectively in molecular weight from bacteria rich in capsule polysaccharides, and doesn＇t need purification for following serial molecular biological experiments.
出处
《土壤》
CAS
CSCD
北大核心
2006年第6期774-777,共4页
Soils
基金
江苏省高校产业化项目(JHB04-017)
江苏省博士后科学基金(0501011B)资助
