摘要
目的扩增和表达人脂联素受体1(AdipoR1)和人脂联素受体2(AdipoR2)基因。方法利用RT-PCR方法扩增AdipoR1和AdipoR2全长cDNA,将AdipoR1和AdipoR2的cDNA构建到真核表达载体pcDNA3.1,重组质粒转染HEK293细胞,通过免疫荧光技术标记HEK293细胞的AdipoR1和AdipoR2融合蛋白,共聚焦显微镜观察AdipoR1和AdipoR2在细胞中的定位。结果成功构建真核表达重组质粒pcDNA3.1-adipoR1和pcDNA3.1-adipoR2,重组质粒转染HEK293细胞后,定位于HEK293细胞膜上。结论AdipoR1和AdipoR2重组质粒的构建和在HEK293细胞中的成功表达,为进一步研究其与胰岛素抵抗的关系创造了条件。
Purpose To clone and express the gene of human AdipoR1 and AdipoR2 in eukaryotic cells. Methods The full-length cDNA of AdipoR1 and AdipoR2 was cloned in pcDNA3.1. The recombinant plasmid was transfected into HEK293 cell to study the expression of AdipoR1 and AdipoR2 in the HEK293 cell. Results pcDNA3. 1-adipoR1 and pcDNA3. 1-adipoR2 were reconstructed successfully. The fusion protein of AdipoR1 and AdipoR2 was localized in the cell membrane of the HEK293 cell. Conclusions The construction of recombinant plasmid and the expression of AdipoR1 and AdipoR2 in the HEK293 cell will be useful to the study on insulin resistance.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2006年第6期845-847,共3页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金项目(编号30470809)资助
