摘要
目的：探讨将Ⅰ型单纯疱疹病毒胸腺嘧啶核苷激酶基因（ＨＳＶ－ｔｋ）／丙氧鸟苷基因治疗系统应用于卵巢癌的治疗。方法：应用产病毒包装细胞（ＶＰＣ）／ＨＳＶ－ｔｋａ和ＶＰＣ／ＨＳＶ－ｔｋｃ的培养液分别感染卵巢上皮癌细胞系ＡＯ和３ＡＯ，以Ｇ－４１８筛选转导阳性克隆，并以丙氧乌苷杀伤阳性克隆。结果：ＶＰＣ／ＨＳＶ－ｔｋｃ培养液对ＡＯ细胞的感染滴度为９．３×１０００００；以１∶１０００稀释度的ＶＰＣ／ＨＳＶ－ｔｋｃ培养液与以１∶１０稀释度的ＶＰＣ／ＨＳＶ－ｔｋａ培养液作转导所获得的阳性克隆数相近；且所有阳性克隆在含有１０μｇ／ｍｌ丙氧鸟苷的培养液中均发生完全死亡。
Objective:Tosearchfortheapplicationoftheretrovirusvector(RV)/herpessimplexvirustypeⅠthymidinekinasegene(HSV-tk)/ganciclovior(GCV)genetherapeuticsysteminthetreatmentofovariancancer.Methods:Twokindsofvector-producingcels(VPC)whichcanpro-duceretrovirusvectorsconstructedtocontainneoRgeneandtwokindsofHSV-tkgenes(markedas“HSV-tka”and“HSV-tkc”)wereemployedtotransferthosegenesintoovariancancerAO,3AOcellines.Andtheresponseofthoseanti-G418clonestoGCVtherapywasobserved.Results:InthetransductiontoAOcels,thevectortiteroftheVPC/HSV-tkcculturewas9.3×100000cfu/ml,andtherewere263anti-G418clonesinthe25cm2celcultureflaskinwhich1∶1000dilutedVPC/HSV-tkcculturewasadded,and235clonesinasimilarflaskinwhich1∶10dilutedVPC/HSV-tkaculturewasadded.Furthermore,aloftheHSV-tkcpositiveclonesexposedto10μg/mlGCVcouldbeeliminated,and96%ofthoseclonesdiedin5daysafterGCVwasused.Conclusions:Theappli-cationoftheRV/HSV-tk/GCVsystemisverypromisinginthegenetherapyofovariancancer.
出处
《中华妇产科杂志》
CSCD
北大核心
1996年第11期664-666,共3页
Chinese Journal of Obstetrics and Gynecology
基金
国家自然科学基金
关键词
卵巢肿瘤
逆转录病毒科
胸苷激酶
OvariancancerRetroviridaeHerpesvirusⅠ
humanThymidinekinase
