摘要
采用５＇加端ＰＣＲ从Ｋ５６２ｃＤＮＡ文库中扩增出含锌指结构域的ｃＤＮＡ基因片段，重组至ｐＧＥＭ７ＺＤＮＡ载体中。通过ＰＣＲ及Ｓｏｕｔｈｅｒｎ印迹杂交初步从Ｋ５６２ｃＤＮＡ文库中筛选出９个含锌指基因片段的重组克隆。经ＤＮＡ序列分析和基因库检索，发现有２个克隆的序列与巳知基因差异显著，有可能为新的锌指蛋白基因片段。
he fragments of cDNA gene coding for zinc-finger proteins were amplified by add-on PCRfrom K562 cells cDNA library.After being cloned into pGEM 7Z Vector,nine of the inserts con-taining zinc-finger construction were identified by PCR and Southern blotting.Two of the recom-binant fragments were found to have many differences in sequences from the known genes by se-quencing and searching for the sequence data in Gene bank.These two recombinant fragmentsmay be cDNA gene fragments coding for new zinc-finger protein。
出处
《湖南医科大学学报》
CSCD
1996年第6期493-496,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金
