摘要
以１次、半巢式及多重ＰＣＲ方法扩增５８例淋巴细胞白血病ＩｇＨ及ＴＣＲＶγＩ－Ｊγ克隆性基因重排。结果显示：多重ＰＣＲ和１次ＰＣＲ的敏感度为１０￣（－４）～１０￣（－５）水平；半巢式ＰＣＲ敏感度可达１０￣（－５）～１０￣（－６）水平。ＩｇＨ和ＴＣＲＶγＩ－Ｊγ重排阳性率为６７．２％和６２．０％；半巢式ＰＣＲ检测ＩｇＨ重排阳性率为７０．７％。上述结果表明：多重ＰＣＲ可同时检测两对目的基因，适于初治病例检测，而对缓解病例的检测则需采用敏感度较高的半巢式ＰＣＲ方法。
We used a series of PCR methods,including one-stage PCR、seminested-PCR and multiprimer-PCR to detect IgH and TCR V γI-Jγ gene rearrangements in 58 lymphoblastic leukemia(ALL and CLL)Patients. The sensitivity of multiprimer-RCR and one-stage PCR was at 10￣(-4)￣10(-6)level,but the sensitivity of seminested PCR was at 10￣(-5)￣10￣(-6)level.67.2% and 62.0% of 58 patients were found to have IgH and TCR V γI-J γ gene rearrangements respectively by one-stage PCR amplification. 70.7% of patients werefound to have IgH gene rearrangement by seminested PCR amplification, Our data show that multipimer-PCR could screen two distinct gene rearrangements in one step PCR amplification and is more suitable forscreening of specimens collected for diagnosis. Seminested PCR is more suitable for detecting minimalresidual disease in patients in complete remission.
出处
《第一军医大学学报》
CSCD
1996年第4期300-302,共3页
Journal of First Military Medical University
基金
国家自然科学基金
全军85卫生医药科研基金
关键词
多聚合酶链反应
白血病
克隆性
基因重排
Polymerase Chain Reaction (PCR)
Leukemia
Clonality Gene Rearrangement
